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Interferon-γ-mediated gene expression in porcine alveolar macrophage; An in vitro model

100%
Liu Q., Wang H.-Y., Liu Q., Wang H.-Y.
Polish Journal of Veterinary Sciences
|
2021
|
vol. 24
|
issue 1
2

EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections

72%
Wang H.-Y., Wei Y.-M., Yan Z.-H., Zheng Y.-L.
Journal of Applied Genetics
|
2007
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vol. 48
|
issue 3
35-42
EN
SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which trinucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.
3

EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections

72%
Wang H.-Y., Wei Y.-M., Yan Z.-H., Zheng Y.-L.
|
EN
SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which tri-nucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.
4

Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf. with reference to wheat breeding

63%
Wang H.-Y., Liu D.-C., Yan Z.-H., Wei Y.-M., Zheng Y.-L.
Journal of Applied Genetics
|
2005
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vol. 46
|
issue 4
365-369
EN
Cytological and agronomic characteristics of a F2 population from Triticum aestivum L. ? T. durum Desf. hybrids were analyzed plant by plant. Means of morphologic traits in the F2 population were similar to those of the low-value parent. On average, F2 hybrids had 36.54 chromosomes per plant, indicating that each gamete lost 2.73 chromosomes at meiosis of the F1 generation. More than half of plants had 36?39 chromosomes, so male gametes with 19?21 chromosomes seemed to be superior to the others. The distribution frequency of chromosomes in this study differed from that in a previous report, where a different tetraploid wheat was used. This shows that a different breeding strategy may need to be taken when exploiting a different tetraploid wheat. According to our results, some plants with 42 chromosomes, having all the wheat A, B and D chromosomes, would appear in the F3 population, which provides a chance to obtain stable bread wheat lines from the self-pollinated progenies. Alternatively, the desirable individuals of the F2 population were backcrossed to bread wheat, which is very useful and efficient for the improvement of bread wheat by exploiting desirable genes in durum wheat.
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