Accurate codon recognition by tRNAs is necessary for correct translation of mRNA nucleotide sequence into the protein sequence. Here, different factors contributing to the correct codon reading by tRNAs are reviewed. In particular, the monitoring of codon-anticodon helix geometry by 16S rRNA bases, and the role of tRNA sequence elements and posttranscriptional modifications for modulating codon-anticodon interactions are discussed.
The article briefly presents ways of RNA isolation from isotopically labelled bacteria and its subsequent degradation to 13C- and 15N-labelled nucleoside 5'-monophosphates then enzymatically converted to NTPs. 13C- and 15N-labelling of RNA is achieved by in vitro transcription with T7 RNA polymerase using isotopically labelled nucleoside 5'-triphosphates as a substrates and DNA template.
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