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1

Plant genes encoding some of the enzymes of phenylpropanoid metabolism pathway

100%

Narozna D., Madrzak C. J.

Biotechnologia

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1999

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issue 3

154-168

EN

The phenylpropanoid metabolism in plants is an important source of numerous compounds of great importance in development and defense. In particular, flavonoids play a significant role as signal compounds, phytoalexins, UV-protectants, pigments etc. Numerous enzymes (PAL, CHS, CHI, CHR, IFR and many others) are involved in this branched metabolic pathway. The key enzymes of phenylpropanoid biosynthesis are phenylalanine ammonia-lyase (PAL), and chalcone synthase (CHS). Both enzymes (as well as some other of the pathway) are encoded by multigene families. The organospecific expression of different members of these families is presumably the key factor for the regulation of the entire pathway. Several cis-elements, and trans-acting factors have been already described, however it is too early to formulate the conclusive model of the overall transcriptional regulation of the phenylpropanoid metabolism.

2

Competition between bacterial strains nodulating leguminous plants

100%

Golinska B., Madrzak C. J.

Biotechnologia

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1999

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issue 3

106-142

EN

Successful nodulation of legumes by rhizobia is a complex process that in open field depends on various environmental and biological factors. Generally legume productivity may be improved by inoculation with selected, highly effective in diazotrophy root nodule bacteria. However, field legume inoculation with Rhizobiaceae species is very often unsuccessful due to the presence of native strains in soil which are better adapted and usually dominate over introduced bacteria. The ability of one strain to outnumber others in nodule occupancy is commonly termed competitiveness. This feature of strain is genetically regulated by numerous bacterial genes, as well as it is highly dependent on host plant genotype and environmental cues. The competitiveness of endogenous strains is critical for the successful use of inocula to introduce the quality strains. In this paper we describe ways and means which should be considered in order to manipulate both established and introduced strains ecologically, edaphically and genetically to improve legume productivity and, as the consequence, soil fertility.

3

Molecular methods for detection, identification and characterization of Fusarium

81%

Golinska B., Narozna D., Madrzak C. J.

Biotechnologia

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2002

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issue 3

165-177

EN

Pathogenic fungi of the genus Fusarium form a diverse, cosmopolitan group of species. Their importance is significant due to the fact that they are responsible for numerous storage rots, plant diseases and human and animal disease caused by Fusarium toxic secondary metabolites. By reason of great extent of variability and plasticity of Fusarium easily accomodating to changing environmental condition, this genus comprises of species that remain very challenging for systematics. Since the reliable and simple detection methods are essential to avoid the losses and health risks, molecular biology methos have been recently implemented for the analysis of Fusarium populations. In this paper, we present a review of selected PCR-based techniques applicale for diagnostic and taxonomic purposes. The RAPD analysis, followed by SCAR-PCR and RFLP method to study fungal rDNA are presented. A number of primer sequences useful for the above methods are also listed.

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3 Biotechnologia

1 2002

2 1999

3 Madrzak C. J.

2 Golinska B.

2 Narozna D.

Plant genes encoding some of the enzymes of phenylpropanoid metabolism pathway

Competition between bacterial strains nodulating leguminous plants

Molecular methods for detection, identification and characterization of Fusarium