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Molecular Monitoring Of Bacterial And Microalgal Biocenoses’ Biodiversity In High Loaded Farming Ponds

100%

Karło A., Ziembińska-Buczyńska A., Pilarczyk M., Surmacz-Górska J.

Ecological Chemistry and Engineering S

2015

vol. 22

issue 3

425-437

Eutrophication process is a serious problem in water ecosystems. There is a great need to study the relation between the physico-chemical condition of water and the influence of these parameters on the diversity of biological life, especially on changes in the structure of microbiocenoses. The most interesting are bacteria and microalgae, due to the important roles they play in maintaining the balance of the aquatic environment. In this study, biodiversity analysis of eukaryotic microalgae and bacteria in two artificial water ecosystems - fish farming ponds - was performed. Aquaculture was based on IMTA technology, in which every part of the trophic chain plays a significant role in maintaining the balance in the ecosystems. Experimental intensive - extensive systems differed in terms of nutrient loads, ponds were characterized by high loads of organic and inorganic nitrogen and phosphorus. During the experimental period, the physicochemical conditions, quantitative genotypic structure of the two biocenoses being studied and the relation between these factors were monitored and investigated. For the biodiversity analysis, the PCR - DGGE technique was used. The results of preliminary research showed that there is a correlation between nutrient loads, diversity expressed in the Shannon-Wiener Index and the overall condition of experimental systems. Higher loadings of nutrient promote the development of bacteria and microalgae without any influence on the balance in the artificial ecosystem being tested.

Start-Up, Modelling and Simulation of the Anammox Process in a Membrane Bioreactor

88%

Cema G., Sochacki A., Kubiatowicz J., Gutwiński P., Surmacz-Górska J.

Chemical and Process Engineering

2012

vol. 33

issue 4

639-650

There are certain well-known methods of diminishing concentrations of nitrogen compounds, but they are ineffective in case of nitrogen-rich wastewater with a low content of biodegradable carbon. Partial nitritation followed by anaerobic ammonium oxidation (Anammox) process appear to be an excellent alternative for traditional nitrification and denitrification. This paper presents the feasibility of successful start-up of Anammox process in a laboratory-scale membrane bioreactor (MBR). It was shown that the combination of membrane technology and Anammox process allowed to create a new highly efficient and compact system for nitrogen removal. It was possible to achieve average nitrogen removal efficiency equal to 76.7 ± 8.3%. It was shown that the start-up period of 6 months was needed to obtain high nitrogen removal efficiency. The applied biochemical model of the Anammox process was based on the state-of-the-art Activated Sludge Model No.1 (ASM 1) which was modified for accounting activity of autotrophs (nitrite-oxidising bacteria and nitrateoxidising bacteria) and anammox bacteria. In order to increase the predictive power of the simulation selected parameters of the model were adjusted during model calibration. Readjustment of the model parameters based on the critically evaluated data of the reactor resulted in a satisfactory match between the model predictions and the actual observations.

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1 Chemical and Process Engineering

1 Ecological Chemistry and Engineering S

1 2015

1 2012

2 Surmacz-Górska J.

1 Cema G.

1 Gutwiński P.

1 Karło A.

1 Kubiatowicz J.

1 Pilarczyk M.

1 Sochacki A.

1 Ziembińska-Buczyńska A.

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Molecular Monitoring Of Bacterial And Microalgal Biocenoses’ Biodiversity In High Loaded Farming Ponds

Start-Up, Modelling and Simulation of the Anammox Process in a Membrane Bioreactor