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1

The influence of DNA isolation's method on result of genetic similarity analysis made by RAPD technique

100%
Dobrzycka A., Broda Z.
Biotechnologia
|
2009
|
issue 3
182-191
EN
The study purpose was to examine the influence of DNA isolation's method on result of genetic similarity analysis made by RAPD technique. Two methods od DNA isolation were used ? the Tomson-Henry method, which provides low purified DNA, and the column method, which provides high purified DNA. By using DNA isolated with these two methods the RAPD analysis of genetic distance was made. It was noticed, that using high purified DNA resulted in higher nu umber of amplification's products, and consequently in larger accuracy of analysis.
2

The induction of haploids of rye (Secale cereale L.) by the anther culture

100%
Broda Z., Mikolajczyk S.
Biotechnologia
|
2001
|
issue 1
148-152
EN
Haploid plants are often used for genome mapping, to induce mutations and also for the production of homozygous plants. They are also needed in heterosis breeding. Androgenesis is one of the methods of their obtainment. The study focused on the development of induction haploid plants and double haploid lines of rye. In the experiment, seven varieties of rye were used: Amilo, Dankowskie Nowe, Dankowskie Z?ote, Kier, Motto, Walet, Warko. The seedlings were vernalised at natural conditions or in the refrigerator. Plants were grown in the greenhouse. Spikes with pollen at the uninucleate stage were harvested and stored at 4 C in the dark for 48 hours. The influence of the varieties and the medium used on the androgenesis was tested. Modified N6 and potato medium were used. Anthers were cultivated in the dark for 4 weeks at 28C. Very low incidence of callus-producing plants (0,0-0,3%) and of any regenerated plants was observed.
3

An attempt at the transformation of embryogenic genotypes of alfalfa using gene pPR97/GUS/Intron-LLpr10.1a promoter

100%
Broda Z., Morawska J.
Biotechnologia
|
2000
|
issue 4
106-111
EN
The transformation was led by pPR97/GUS/Intron LLpr10.1a promoter gene, which codes the acid protein. Two different media were used in the experiment. On Blayds medium, the highest efficiency of soamatic embryogenesis (25%) was observed in the Kometa variety. The culture media developed by Guelph University appeared to be the best for the Kama variety (18%).
4

Regeneration efficiency evaluation of selected rapeseed (Brassica napus ssp. oleifera) doubled haploid lines

100%
Turczynowska A., Broda Z.
Biotechnologia
|
2010
|
issue 2
132-138
EN
Regeneration is an important step in a process of obtaining valuable plants under in vitro conditions. The aim of the research was to characterize the regeneration efficiency of 14 rapeseed (Brassica napus ssp. oleifera) doubled haploid lines which were supposed to reveal different tissue response under in vitro conditions. The plant material consisted of cotyledonous and hypocotylous explants placed on MS medium with the addition of 1mg/l and 0,1mg/l BAP. Regeneration efficiency evaluation was calculated on the basis of shoot regenerating explants number in relation to the total number of explants. The examined genotypes were characterized by varied regeneration ability and closely related DH lines with high and low regeneration efficiency were determined.
5
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In vitro cultures of carnivorous plants from the Drosera and Dionaea genus for the production of biologically active secondary metabolites.

81%
Mikołajczyk S., Broda Z., Weigt D.
BioTechnologia
|
2012
|
issue 2
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