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Fibronectin fragments in human seminal plasma.

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Kątnik-Prastowska I., Przybysz M., Chełmońska-Soyta A.

2005

vol. 52

issue 2

557-560

The study has revealed the presence of fibronectin (FN) fragments and a lack of intact FN in 72 seminal plasma samples. The FN fragmentation was examined by immunoblotting with a monoclonal antibody specific to the central cellular FN domain and was confirmed with a monoclonal antibody directed to the C-terminal domain of FN. Nine FN fragments between 60 and 200 kDa and five fragments of 60-150 kDa were identified in seminal plasma samples of normozoospermic and of terato-, oligoterato-, and oligoasthenoterato-spermic groups, respectively. The relative amounts of the 60, 90 and 100 kDa FN fragments were 2-3 times higher in seminal plasmas with abnormal semen characteristics than in the normozoospermic group. The results suggest that seminal plasma FN fragments may contribute to fertilization and the analysis of FN fragmentation may have a diagnostic value in andrological investigations.

Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

100%

Pupek M., Lemańska-Perek A., Jasonek J., Kątnik-Prastowska I.

2010

vol. 57

issue 3

333-337

Three monoclonal antibodies specific to the central cell-binding and the C- and N-terminal domains of fibronectin (FN) were used to test antigenic epitope accessibility on human plasma and cerebrospinal fibronectins. In the plasma group, the mean N-terminal FN domain immunoreactivity was about one fourth that of the cell-binding and C-terminal domains, whereas in cerebrospinal fluid they were nearly equal. In the presence of 0.5-6 M urea N-terminal domain immunoreactivity in the plasma increased 3-6-fold, but it decreased 0.7-3-fold in the cerebrospinal fluid. Analysis of fibronectin domain immunoreactivities of the cell-binding and N-terminal domains by a panel of specific monoclonal antibodies may reveal N-terminal fibronectin domain accessibility for reaction with biological partner ligand(s) and/or processes in which FN could be implicated. Such determinations may have important clinical implications.

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2 Acta Biochimica Polonica

1 2010

1 2005

2 Kątnik-Prastowska I.

1 Chełmońska-Soyta A.

1 Jasonek J.

1 Lemańska-Perek A.

1 Przybysz M.

1 Pupek M.

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Fibronectin fragments in human seminal plasma.

Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains