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ALLIUM URSINUM L. LEAVES COMPONENTS MODIFIED THE PHYSICO-CHEMICAL PROPERTIES OF RED BLOOD CELLS PROTECTING THEM FROM THE EFFECTS OF OXIDATIVE STRESS

100%
Cyboran-Mikołajczyk S. M., Kleszczyńska H., Oszmiański J., Pasławski R.
Acta Poloniae Pharmaceutica - Drug Research
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2019
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vol. 76
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issue 3
483-491
EN
The aim of the study is to determine the antioxidant activity and influence of Allium ursinum L. leaf extract on physicochemical and electrical properties of erythrocytes in order to help clarify its capacity to alleviate oxidative stress. The phenolic compounds were isolated from the leaves of bear garlic. The composition of the extract is well documented and was earlier analyzed quantitatively and qualitatively by UPLC methods. The impact of the extract constituents on the erythrocyte membrane properties was determined on the basis of its osmotic fragility, fluidity, organization of the hydrophilic area and transmembrane potential with spectrophotometric and fluorimetric methods. Furthermore, the effect of the extract on the level of membrane lipid oxidation and also on the extent of hemolysis of erythrocytes subjected to oxidation induced by AAPH compound was determined. The study showed that the extract at 40 µg/mL lowers the level of erythrocyte hemolysis caused by 76.8% oxidation. In addition, the extract protects lipid membranes against hemolysis better than ascorbic acid. The extract, in examined range of concentrations, did not induce hemolysis of erythrocytes, caused small decrease in their electric potential and osmotic fragility, and increased disorder of the polar heads of membrane lipids. The high antioxidant efficiency and determined effects of the interaction of bear garlic leaf extract with the erythrocyte membrane suggest that its constituents may not only protect cells against free radicals but also mitigate the effects of oxidative stress.
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A micelle mediated extraction as a new method of obtaining the infusion of Bidens tripartita

88%
Śliwa K., Sikora E., Ogonowski J., Oszmiański J., Kolniak-Ostek J.
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2016
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vol. 63
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issue 3
543-548
EN
In this paper, the extracts of three-part beggarticks (Bidens tripartita) were prepared using ultrasonic assisted micelle-mediated extraction method (UAMME). Different kind of surfactants and WPC (whey protein concentrate) were applied. In the B. tripartita extracts twenty polyphenols were identified by UPLC-DAD-MS. Both aqueous and UAMME extracts mainly contained chlorogenic acid, caftaric acid and its derivatives as well as luteolin 7-O-glucoside. The luteolin was extracted with Rokanol B2 and Triton X-100. Furthermore, antioxidative properties of the extracts were analyzed with two methods: reactions with di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) reagent and Follin's method. The DPPH radical scavenging by micellar extracts was in general comparable with the antioxidant activity of conventional extracts. The most active was sample with Tego Care CG90, reducing about 73% of the radical. Obtained results confirmed that the UAMME might be an alternative method, to the liquid-liquid or solid-liquid extraction, of obtaining specified extracts rich in active compounds. Selecting a suitable surfactant may thus provide the expected composition of the extract.
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