In this study, the apparatus for continuous controlling of dissolved oxygen concentration in the culture media was used. The automatic dosing of hydrogen peroxide (decomposed to oxygen and water) was possible, and a significant increase of extracellular enzymatic activity of inulinase (2,5-fold) and invertase (1,5-fold) were obtained in comparison with the traditional aeration. It also minimizes contamination, lowers the expenses on power consumption on aeration and mixing, reduces foaming and, consequently, high cost of antifoam emulsion and bacteriological filters.
Studies described in this paper were conducted in order to determine the dependence between the conditions of ligninocellulosic substrate pretreatment and its suceptibility to enzymic hydrolysis. In view of this, a semi-empirical mathematical model was used to examine the process of enzymic hydrolysis of cellulose by assuming that this reaction proceeds as a sum of two first order reactions with different rates of reactions. The application of quasi-Newtonian equations allowed for the determination of percentage fractions of easily and non-easily hydrolised cellulose in the structures of pretreated substrates.
A new apparatus for controlling dissolved oxygen concentration in the culture media was tested. Using the apparatus, the automatic dosing of hydrogen peroxide (decomposed by catalase to oxygen and water) was possible, and a significant increase of dissolved oxygen, gluconic acid (3-fold) and over 5-fold intracellular catalase activity were obtained in comparison with the traditional aeration. It also minimises contamination, lowers the expenses on power consumption of aeration, reduces foaming and, consequently, high cost of antifoam emulsion and bacteriological filters.
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