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1

Identification of acidic pathogenesis-related (PR) proteins in potato leaves infected with potato virus Y

100%
Marczewski W.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 3
251-253
2

AFLP - a new method in plant genetics

100%
Marczewski W.
Biotechnologia
|
1997
|
issue 2
121-126
EN
A new method called AFLP is presented. The technique involves three steps: restriction of the genomic DNA and ligation of adapters, selective PCR amplification of the restriction fragments, identification of the amplified restriction fragments. The AFLP method is a very powerful DNA fingerprinting technique for DNAs of any origin or complexity.
3

Identification of RAPD markers in plants

100%
Marczewski W.
Biotechnologia
|
1999
|
issue 1
106-115
EN
The random amplified polymorphic DNA (RAPD) method is based on random amplification of DNA fragments, via PCR, using short primers of arbitrary sequence. RAPD markers have been applied to construct linkage maps, to assess genetic diversity, to study taxonomic relationships, and to tag disease resistance genes in plants. RAPD markers linked to a resistance gene can be identified using bulked segregant analysis (BSA), recombinant inbred lines (RILs) or near-isogenic lines (NILs). More reliable and specific PCR-based markers known as sequence-characterized amplified region (SCAR) and allele-specific associated primer (ASAP) were developed. There are several examples of the application of these DNA marker systems in marker-assisted plant breeding.
4

Inter-simple sequence repeat (ISSR) markers for the Ns resistance gene in potato (Solanum tuberosum L.)

100%
Marczewski W.
|
|
issue 2
139-144
EN
Inter-simple sequence repeat (ISSR) polymorphism was used for finding markers linked to the Ns gene, responsible for a resistance of potato (Solanum tuberosum L.) to potato virus S (PVS). The ISSR markers UBC811660 and UBC811950 were found to be linked to Ns. Linkage distances were estimated to be 2.6 cM and 6.6 cM, respectively. UBC811660 showed high accuracy for detection of PVS resistance in diploid potato clones. In tetraploids, among seventeen studied genotypes containing the resistance gene, this marker was revealed in eleven. UBC811660 can be a powerful tool for detection of genotypes carrying the Ns gene in diploid potato breeding programmes.
5

Biotechnology in potato breeding

100%
Marczewski W.
Biotechnologia
|
2008
|
issue 2
20-26
EN
Potato has been known in Europe for the past 400 years. Potato breeding began after the global Phytophthora infestans epidemics in 1840 and 1841. The first breeding attempts were to increase the P. infestans resistance in cultivated potato. In the 21st century, new potato cultivars have been evaluated for more than 50 traits, both for disease and pest resistance, and significant tuber traits. Modern potato breeding requires biotechnology. Cell and tissue culture, genetic engineering and DNA-based molecular markers are the most promising areas of molecular biology for potato breeding. However, there are few reports indicating the actual use of biotechnology in potato breeding programs. Cisgenesis and DNA markers that reside within resistance genes or physically close to them and identified in a fully automated system are a chance for the progress in the future.
6

Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars

45%
Szajko K., Chrzanowska M., Strzelczyk-Zyta D., Zagorska H., Marczewski W.
Journal of Applied Genetics
|
2008
|
vol. 49
|
issue 1
45-47
EN
The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811454 revealed additional amplicons with a similar electrophoretic migration rate as that of SC811454, which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.
7

Genetic relationship between Polish and Chinese strains of the mushroom Agaricus bisporus (Lange) Sing., determined by the RAPD method

39%
Staniaszek M., Marczewski W., Szudyga K., Maszkiewicz J., Czaplicki A., Gian G.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 1
43-47
EN
The genetic relationship between twenty-six strains of Agaricus bisporus were analysed by the RAPD (random amplified polymorphic DNA) method. DNA amplification was performed with the use of twelve arbitrary 10-mer primers. Four primers, which gave polymorphic band patterns were chosen for RAPD analysis. In total, they gave 24 distinguishable bands, of which nine were polymorphic. The conducted research showed that there is a great genetic similarity among the examined strains. Low polymorphism of the strains may be a proof of a limited genetic pool used in the cultivation of those strains.
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