Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 3

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Field performance of transgenic clones obtained from potato cv. Irga

100%
Flis B., Zimnoch-Guzowska E.
Journal of Applied Genetics
|
2000
|
vol. 41
|
issue 2
81-90
EN
The aim of the study reported here was to evaluate the performance and variability of transgenic potato clones. For the genetic transformation the Polish potato cultivar Irga was chosen in order to improve resistance to a necrotic strain of potato virus Y (PVYN ) by introducing to its genome a truncated gene coding PVYN replicase (in sense and antisense orientations). Transgenic plants and clones derived from them were propagated and the obtained tubers were planted in a replicated field trial. The several agronomic and morphological traits were evaluated and compared with measurements for non-transgenic control plants. The traits of transgenic clones showed a much greater variability than non-transgenic plants. The variability depended on the type of the introduced construct (in this case it was the orientation of the construct). None of the transgenic clones turned out to be completely true to type and resistant to PVYN, but some resistant clones expressed deviations in a small proportion of the traits. Usually, deviations were observed for those traits whose inheritance is characterised by a large environmental component. Genetic transformation is an effective method for introducing resistance. However, the method causes a great variability, which makes selection among transgenic clones a necessary step in breeding of an improved transgenic cultivar. Such selection has many similarities with selection done among traditionally obtained clones.
2

Resistance to Phytophthora infestans in diploid and tetraploid potato families. 2. Resistance in tubers

71%
Swiezynski M., Flis B., Osiecka M., Sieczka M. T., Zarzycka H.
|
|
vol. 38
|
issue 1
33-44
EN
The tuber reaction to Phytophthora infestans was evaluated in individual genotypes of four diploid and four tetraploid potato families, their parents and standard cultivars. The genotypes were tested several times. Two testing methods were used, based on evaluation of tuber slices or whole tubers, respectively. All inoculations were done with MP 245, a fungus isolate with a broad virulence spectrum. If the reaction of tuber slices was evaluated, the distribution of genotypes in several families deviated considerably from a normal one. In some distributions more than one peak was detectable, indicating segregation of major genes. Genotypes transgressive in resistance were found in four families. If the reaction of whole tubers was evaluated, the distribution of genotypes did not deviate significantly from a normal one. Genotypes transgressive in resistance were found in one family. The tuber reaction to P. infestans depended to a large extent on testing conditions.
3

Potato and tobacco cultivars transformation towards potato virus Y resistance

61%
Chachulska A. M., Chrzanowska M., Flis B., Krzymowska M., Lipska-Dwuznik A., Robaglia C., Zagorski W.
Biotechnologia
|
1997
|
issue 4
48-54
EN
In recent years a new subgroup of necrotic potato virus Y (PVY) isolates has spread in the Polish fields. To counter this infection, the PVY resistance was introduced into selected potato and tobacco cultivars. Plants were transformed by agroinfection with pROK2 derived binary plasmids, carrying an appropriate fragment of the PVY genome (patent applied for). Kanamycin resistant plants were screened by PCR for the presence of PVY cDNA inserts, positive transformants were tested for virus resistance by inoculation with sap from infected plants, followed by observations of disease symptoms and virus accumulation analysis. Several resistant potato and tobacco clones were identified and their phenotypes were preliminarily characterised.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.