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PL
Białka frakcji cytosolowej oraz frakcji błon plazmatycznych komórek łagodnego rozrostu stercza oraz raka tego gruczołu analizowano za pomocą jednokierunkowej elektroforezy w żelu poliakryloamidowym z SDS (PAGE-SDS). Prawidłową tkankę stercza, łagodny rozrost (BPH ang. benign prostatic hyperplasia) oraz raka prostaty (PCA, ang. prostatic carcinoma) pozyskiwano w wyniku częściowej elektroresekcji przezcewkowej lub całkowitej prostatektomii. Rozdziały elektroforetyczne białek wybarwiano metodą srebrową a następnie analizowano za pomocą wideodensytometru i programu komputerowego Gel-Pro® Analyzer (Media Cybernetics, USA). Nie stwierdzono istotnych zmian jakościowych i/lub ilościowych w obrębie białek frakcji błon plazmatycznych badanych tkanek. We frakcji cytosolowej prawidłowego stercza, BPH i PCA zaobserwowano natomiast różnice natury zarówno jakościowej jak i ilościowej. Znaczący w przypadku raka prostaty, w porównaniu z tkanką prawidłową i BPH, wzrost ekspresji białka 32 kD wydaje się być specyficzny dla nowotworu złośliwego. Z kolei zmiany ilościowe białek 62 kD i 93 kD stwierdzone w BPH jak i PCA sugerują podobny przebieg wczesnych etapów karcynogenezy obu wymienionych nowotworów.
EN
Study aim: To assess the physical activity of male and female adolescents from Poland living in a town (Czechowice-Dziedzice) and in a city (Katowice).Material and methods: The research involved 431 high school students aged 16-18 years (249 from Czechowice-Dziedzice and 182 from Katowice). Physical activity was measured by the short form of the International Physical Activity Questionnaire (IPAQ). The volume of the different levels of physical activity was calculated in units of MET min/wk.Results: The total volume of weekly physical activity was higher among high school students living in a town (Czechowice-Dziedzice) than students living in a city (Katowice). Only 31.5% of the students met the criteria for recommended physical activity.Conclusions: The level of physical activity varies by place of residence. Students living in towns are more physically active than youth living in cities. Most high school students (64%) included in the research can be considered to be insufficiently physically active.
EN
Backround: The aim of this study was the assessment of energy expenditure (EE) and the intensity of physical activity (PA) of adult women during the interactive dance video game (IDVG) Dance Central on Xbox 360 Kinect in the context of health recommendations. Methods: Twenty eight intentionally selected women (mean ± SD age: 21.8±1.1 years, body height: 167.3±6.1cm, body mass: 59.3±5.9 kg, body mass index: 21.2±1.3) met the inclusion criteria and took part in to the experiment. Heart rate monitor, accelerometer, and pedometer were used as measurement tools. Participants performed the same easy dance routine three times (for a total of 10 min.). Trials consisted of imitating the motions presented by the virtual dancer. Results: The data from accelerometer and heart rate monitor were similar, and indicated the moderate intensity of interactive dance video game (IDVG) Dance Central. However, energy expenditure assessed by pedometer was significantly lower. Conclusion: IDVG Dance Central may be useful in increasing the daily dose of physical activity of adult women and meet the health-related recommendations provided its regular practice.
EN
Study aim: the objective was to assess and compare the energy expenditure (EE) and exercise heart rate (EHR) during Nordic Walking (NW), and conventional walking (W) in physical education and tourism/recreation university students. Material and methods: a total of 53 women and 65 men, students at the Academy of Physical Education in Katowice, and Polytechnic in Opole, in Poland, were investigated to assess the EE of Nordic Walking and conventional walking, and to measure the EHR, a uniaxial accelerometer – Caltrac Monitor, and a pulsometer – Polar RS 400 SD were used. After a standard warm-up, the participants (joined group) were marching with poles for 30 minutes, at a speed regulated by the group’s leader, who used footpod’s indications: 5.5–6.5 km/h – in Katowice, and 7.5–8.5 km/h – in Opole. After a break, during which the participants’ heart rate decreased below 100 bpm, the above actions and measurements were repeated, during normal walking. Results: energy expenditure during Nordic Walking (EE NW), expressed in kcals and METs, was in women and in men, at both analyzed speeds, significantly higher (p < 0.05) than during conventional walking (EE W). EHR was higher during NW, compared to W, and the statistically significant differences were revealed both in female’s and male’s groups. Conclusions: our study data have shown that EE and EHR during Nordic Walking, have been significantly higher than during conventional walking, regardless of the moving speed and gender. NW might present a useful modality in prevention of hypokinetic disorders.
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