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1

Vitrification of mammalian oocytes and embryos

100%
Smorag Z., Gajda B.
Biotechnologia
|
1995
|
issue 3
68-83
EN
Vitrification is a new approach to oocyte and embryo cryoconservation.It consists in the solidification of a solution caused by draastic increase in viscosity during cooling and not by crystalization.The application of this approach to cryoconservation of oocytes and embryos of different species depends upon the development of proper procedures and non-toxic media.From the technical point of view, the vitrification method is simple and relatively easily applicable under field conditions.The authors review the current procedures applied to oocytes and embryos of laboratory and farm animals.
2

Cryopreservation of porcine oocytes and embryos

100%
Gajda B., Smorag Z.
Biotechnologia
|
2003
|
issue 1
116-228
EN
This paper presents the current possibilities, state of knowledge and prospects for cryopreservation of pig oocytes and embryos. The main factors of cryopreservation efficiency, methods for the evaluation of cryopreserved embryos, and the possibilities of modifying their susceptibility to cryopreservation are discussed. In addition, the most significant results of pig embryo freezing and vitrification and the cryotechnical aspects of this method are presented.
3

Application of reproduction biotechnology methods in animal biodiversity conservation

100%
Gajda B., Smorag Z.
|
2007
|
issue 4
55-65
EN
This article presents the potential and prospects for the use of selected reproduction biotechnology methods in animal biodiversity conservation programs. The first part focuses on biotechnological methods related to male reproduction such as artificial insemination, semen cryoconservation, sexing and spermatozoa sorting. The second part discusses biotechnological methods related to female reproduction such as superovulation, in vitro production and transfer of embryos, cryoconservation of oocytes and embryos, embryo sexing, cloning and transgenesis.
4

Cryoconservation of mammalian embryos and oocytes

100%
Gajda B., Smorag Z.
Biotechnologia
|
1998
|
issue 2
10-33
EN
This paper presents current methods of embryo and oocyte cryoconservation in the following species: mice, rabbits, sheep and goats, pigs, horses and cows. Both the freezing and vitrification methods are discussed with special emphasis on the major factors affecting the efficiency of these two methods.
5
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Influence of H2O2 and O3 on PGM Extraction from Used Car Catalysts

81%
Gajda B., Fornalczyk A., Willner J., Sedlakova-Kadukova J.
Archives of Metallurgy and Materials
|
2018
|
vol. 63
|
issue 2
6
Publication available in full text mode
Content available

Mathematical and Physical Modeling of Alloy Behavior Feeding by Pulse-Step Method to Liquid Steel in One Strand Slab Tundish

81%
Cwudziński A., Gajda B., Hutny A., Jowsa J.
Archives of Metallurgy and Materials
|
2018
|
vol. 63
|
issue 4
7

Effect of protein source, witamin E and phenazine ethosulfate on developmental competence and quality of porcine embryos cultured in vitro

81%
Gajda B., Baryla M., Smorag Z.
Folia Biologica
|
2008
|
vol. 56
|
issue 1-2
57-63
EN
Effects of fetal calf serum (FCS) or bovine serum albumin (BSA), with or without vitamin E (vit. E) or phenazine ethosulfate (PES) supplementation on developmental competence and quality of cultured porcine embryos were examined. The experiment was done on zygotes and 2-cell embryos obtained from superovulated gilts. Morphologically normal zygotes were cultured in vitro in NCSU-23 medium supplemented with: experiment 1-0.004 g/ml BSA, 10% FCS, protein-free (control); experiment 2-0 (control), 25, 50 or 100 M vit. E; experiment 3-0 (control), 0.025, 0.05 or 0.075 M PES. Embryo quality criteria were developmental competence (cleavage, morula and blastocyst rates), total cell number per blastocyst and degree of apoptosis as assessed by TUNEL staining. Presence of BSA in culture medium increased significantly morula and blastocysts production as compared to FCS (P<0.001) and protein-free group (P<0.05 and P<0.001, respectively). The blastocysts cultured in protein-free medium had higher average number of apoptotic nuclei and DNA fragmented nucleus index as compared to the BSA (P<0.05 and P<0.01, respectively) and FCS (P<0.5) group. Supplementation in culture medium of 100 M vit. E increased blastocyst production as compared to control and 50 M vit-E (P<0.05). Both the number of cells per and percentage of TUNEL positive nuclei per blastocyst were slightly lower in PES treated than control groups.
8

Transgenic pigs: production and genetic modification

62%
Smorag Z., Jura J., Kopchick J. J., Gajda B., Skrzyszowska M., Rozycki M., Pasieka J.
Biotechnologia
|
1998
|
issue 2
145-152
EN
This paper presents the methods of transgenic pigs production and the results based on the long experience of the authors in this area. Moreover, the trends and current issues of transgenic modification in pigs are discussed.
9

Factors affecting production of transgenic farm animals: cattle and rabbits

62%
Jura J., Smorag Z., Katska L., Skrzyszowska M., Gajda B., Rynska B.
Biotechnologia
|
1998
|
issue 2
101-109
EN
There are many factors affecting transgenic farm animals production. One of them is the effectiveness of the transfer of zygotes and embryos obtained after DNA microinjection. Low effectiveness of the transfer of potentially transgenic blastocysts in cattle is due to their decreased developmental potential in comparison to the blastocysts developed from not microinjected zygotes. A simple short term in vitro culture used for rabbit zygotes after microinjection increased twice the number of produced potentially transgenic rabbits.
10

The expression of WAP-Fuc gene in genotypes of transgenic farm animals ? effectiveness of transgenesis with the use of DNA microinjection

62%
Jura J., Smorag Z., Gajda B., Kareta W., Kopchick J. J., Kelder B., Prieto P. A.
Biotechnologia
|
2003
|
issue 1
216-222
EN
There are two principal applications of transgenic animals. Best known and most advanced application is to use transgenic animals (called bioreactors or molecular farms) for the production of various proteins or biopolymers of medical significance. The second application concerns efforts to improve the productivity traits of breeding animals. We have worked out methods which allow somatic cloning of mammals and gene knockout methods. These methods have been developing very rapidly in recent years and their efficiency will soon be improved to the extent that they will become profitable. For the time being, DNA microinjection with all its disadvantages, remains the principal method of producing transgenic bioreactors. In this paper, the effectiveness of production of transgenic rabbits, goats and pigs with the use of WAP-Fuc gene construct is presented.
11

Performance of transgenic pigs produced with the use of two different growth hormone gene constructs

52%
Rozycki M., Smorag Z., Kopchick J., Chen W. Y., Jura J., Pasieka J., Orzechowska B., Gajda B., Skrzyszowska M.
Journal of Applied Genetics
|
1999
|
vol. 40
|
issue 1
29-37
EN
Four transgenic pigs, produced with the use of two different gene constructs containing the bovine growth hormone coding gene - Mt-bGH-10D6 (wild type) and Mt-bGH-M8 (mutated), were used to produce the F1 generation to investigate their performance traits. No differences were observed in fattening and slaughter traits between transgenic and non-transgenic pigs. It was found that the weight of ham proper and loin eye area was significantly higher in carriers of Mt-bGH-10D6 gene constructs.
12

Production of pigs used in xenotransplantation

52%
Jura J., Slomski R., Smorag Z., Gajda B., Wieczorek J., Lipinski D., Kalak R., Juzwa W., Zbyland J.
Biotechnologia
|
2006
|
issue 1
151-158
EN
There are four main trends in the use of transgenic animals. One of the most interesting is the use of transgenic animals as the donors of tissue or organs suitable for transplantation in human ? xenotransplantation. This field of research has been undergoing intensive and increasing study during the past few years, and some encouraging progress is being made. A pig has been identified as the most suitable donor animal. The aim of the presented experiment was to produce transgenic pigs which tissues and organs could be used for the xenotransplantation needs. To target the goal, a competitive gene construct coding the same substrate as the endogenous enzyme of organ donor was introduced. In effect, transgenic boar was produced with confirmed integration of human a1,2 fucosyltransferase gene. Also, F1 generation of transgenic pigs was generated to preserve ongoing needs of preliminary research of xenotransplantation project.
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