Nitric oxide (NO) is increasingly being used in medical applications. Currently, a gas cylinder of N_{2} mixed with a high concentration of NO is used in the NO inhalation system. However, this arrangement is potentially risky due to the possibility of accidental leak of NO from the cylinder. The presence of NO in air leads to the formation of nitric dioxide (NO_{2}), which is toxic to the lungs. Therefore, an on-site generation of NO would be very desirable for patients with acute respiratory distress syndrome and other related illnesses. Previously, our group reported the production of NO using a pulsed arc discharge. In this work, the prototype of the on-site NO generator was developed and the performances of the NO generator were demonstrated for medical applications.
Retrotransposons are mobile genetic elements that accomplish transposition via an RNA intermediate. These elements can be transcriptionally activated by stress factors, such as UV light, ozone, pathogens, wounding and drought. A red alga, Porphyra yezoensis has recently been recognized as a model plant for fundamental and applied study in marine biological science. In this paper, pulsed streamer-like discharge in liquid was used as a new stress condition, and the transcriptional levels of retrotransposon and functional genes of a red alga, Porphyra yezoensis was investigated. From the results of reverse transcription-polymerase chain reaction (RT-PCR) experiments, it is clear that the transcriptions of retrotransposon and functional genes were activated by applying pulsed streamer-like discharge.
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