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Understanding Radiation Damage to Cells Using Microbeams

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Folkard M., Prise K., Shao C., Gilchrist S., Schettino G., Michette A., Vojnovic B.
Acta Physica Polonica A
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2006
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vol. 109
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issue 3
257-264
EN
Cellular micro-irradiation techniques provide unique experimental opportunities for understanding how ionizing radiation interacts with living cells and tissues. Using microbeams, it is possible to deliver precise doses of radiation to selected individual cells, or sub-cellular targets in vitro. This technique continues to be applied to the investigation of a number of phenomena currently of great interest to the radiobiological community. In particular, it is the study of so-called "non-targeted" effects (where cells are seen to respond indirectly to ionizing radiation) that are benefiting most from the use of microbeam approaches.
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Development of a Soft X-ray Microprobe for Radiobiology Studies

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Appuhamilage I., Adjei D., Alatabi S., Alnaimi R., Michette A., Pfauntsch S.
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vol. 125
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issue 4
882-885
EN
The King's College London (KCL) first X-ray microprobe (MKI) and the third generation microfocus X-ray sources (MKIII) are intended to be used for various applications including the study of physical and biological interactions at the atomic and molecular scales. The microfocus ultra-soft X-ray sources (MKI and MKIII) with interchangeable targets will provide a superior spatial resolution (a focal spot a few hundreds of nanometres in diameter can be achieved) and the control of the dose delivered to irradiated cells. This will require characterization of the spectra and intensities of the source, measurements of the focus intensities and spot sizes of suitable X-ray optics such as zone plates, grazing incidence microstructured optical arrays and multilayer mirrors.
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