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PREPARATION OF NANOCRYSTALS AND CHITIN MICROFIBRILS

100%
Biniaś D., Biniaś W.
Progress on Chemistry and Application of Chitin and its Derivatives
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2022
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vol. 27
43-53
EN
Chitin nanocrystals and microfibrils were prepared based on the strong acid hydrolysis method. The change in the molecular structure was studied by Fourier-transform infrared and Raman spectroscopy. The surface morphology of chitin materials was observed using scanning electron and optical microscopy. The separation of nano-crystals and microfibril crystalline regions from native chitin may increase the scope of its application for the creation of bioactive composites and scaffolds for tissue culture. As a result of the conducted experimental work, it was possible to loosen the amorphous areas and partially separate nano-crystals and chitin microfibrils. Stable aqueous suspensions of chitin nano-crystals and microfibrils were obtained, which can be used for further experimental work.
2
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FORMATION OF FIBRES AND SPHERES FROM CHITIN SOLUTION

81%
Biniaś D., Biniaś W., Janicki J.
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vol. 23
25 - 32
EN
The applications of chitin are limited due to its insolubility in most organic solvents. The chemical modification of chitin to generate new bio-functional materials can bring more desirable properties. A concentration of 5% can be obtained. We have successfully prepared chitin fibres and spheres by dissolving chitin in 85% phosphoric acid in various coagulating agents and then regenerating it in 10% sodium hydroxide. The change in molecular structure was studied by Fourier Transform Infrared Spectroscopy (FTIR). The surface morphology of different biomaterials was observed using scanning electron microscopy (SEM) and optical microscopy (OM).
3
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STRUCTURAL RESEARCH OF THERMOSENSITIVE CHITOSAN-COLLAGEN GELS CONTAINING ALP

81%
Skwarczyńska A., Biniaś D., Modrzejewska Z.
Progress on Chemistry and Application of Chitin and its Derivatives
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2016
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vol. 21
176-186
EN
Introducing collagen, which is basic ingredient of bone tissue, into the structure of chitosan gels which are formed at the physiological body temperature, is aimed at creating the so-called biomimetic structures, i.e. close in their composition to the natural composition of bone tissue. Within the research the influence of collagen on structural properties of thermosensitive chitosan gels and the influence of ALP on structural properties of chitosan and chitosan-collagen gels was determined.
4
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FORMATION AND PROPERTIES OF DBC/PLA MICROFIBRES

71%
Biniaś D., Biniaś W., Machnicka A., Janicki J., Hałuszka P.
Progress on Chemistry and Application of Chitin and its Derivatives
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2017
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vol. 22
5-13
EN
In this study, a solution of dibutyrylchitin (DBC)/polylactide (PLA) blend micro and nanofibres were successfully fabricated using blends of 2,2,2-trifluoroethanol (TFE) as solvents. Fibres were produced from the solutions by electrospinning. The DBC/PLA blend solutions in various ratios were studied for electrospinning into micro/nanofibres. The morphology of the micro and nanofibres was observed by scanning electron microscope (SEM). The biggest diameters of DBC/PLA fibres were obtained for the blended microfibres in ratios of 10/90 and 25/75. The smallest diameter was observed for pure polymers. The antibacterial properties were examined for materials obtained by electrospinning. In the experiments, materials with antibacterial properties were made. It is likely that the electrospun micro and nanofibres will be used in the native extracellular matrix for tissue engineering.
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