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Long-lasting effects of social stress on peritoneal inflammation in some strains of mice

100%

Scislowska-Czarnecka A., Chadzinska M., Pierzchala-Koziec K., Plytycz B.

Folia Biologica

2004

vol. 52

issue 1-2

97-104

Adult male mice were kept for one week either one or four animals per cage. Some were maintained under the same social conditions for an additional 9 days (controls); their counterparts were either grouped (4 per cage) or isolated (1 per cage). Changes in housing conditions caused a significant increase of plasma corticosterone measured 30 minutes after separation or grouping of SWISS, C57C3H, and BALB/c but not of C57BL/6 mice. Peritoneal inflammation was induced by i.p. zymosan injection on day 9 after changes in housing conditions when corticosterone was again at its initial level in each group. Peritonitis-connected pain symptoms, exudatory PMN numbers, and cytokine (IL-1_ and MPC-1) and corticosterone levels were compared between animals living in stable social conditions with those shifted 9 days earlier from separation to the group or vice versa. These factors were unaffected by social stress in C57BL/6 mice and in SWISS animals transferred from the group to isolation. In all other instances at least two parameters were significantly different in the post-stressed and control animals, being either enhanced or inhibited. In conclusion, social stress had long-term consequences on the course of inflammation in three out of four investigated strains of mice.

Effects of macrophage depletion on peritoneal inflammation in swiss mice, edible frogs and goldfish

88%

Chadzinska M., Kolaczkowska E., Scislowska-Czarnecka A., Van_ R. N., Plytycz B.

Folia Biologica

2004

vol. 52

issue 3-4

225-231

SWISS mice, edible frogs and goldfish i.p. injected with zymosan (Z groups) develop peritoneal inflammation connected with a massive intraperitoneal accumulation of leukocytes, which is significantly diminished in mice and fish (but not frogs) by supplementation of zymosan with morphine (ZM groups). In order to check the putative role of resident peritoneal macrophages in morphine-modulated zymosan-induced peritonitis, some animals were depleted of resident macrophages by repeated i.p. injections of clodronate-liposomes (CL) followed by Z or ZMinjection. In SWISS mice such CL-induced removal of Mac-3-positive cells (macrophages) resulted in an enhanced influx and prolonged accumulation of polymorphonuclear leukocytes (PMNs) in CL-Z and CL-ZM groups in comparison with their counterparts with intact macrophages. Nevertheless, supplementation of zymosan with morphine inhibited the early stages of peritonitis in CL-treated animals as it did in untreated mice. This indicates that intact peritoneal macrophages of SWISS mice are important for limiting PMN accumulation, perhaps mainly through the release of IL-10, but are not critical for the induction of anti-inflammatory effects of morphine during the early stages of peritonitis. Unexpectedly, macrophage depletion in CL-treated frogs and fish resulted in a lack of a typical peritonitis in both Z and ZM groups of these ectothermic animals.

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2 Folia Biologica

2 2004

2 Chadzinska M.

2 Plytycz B.

2 Scislowska-Czarnecka A.

1 Kolaczkowska E.

1 Pierzchala-Koziec K.

1 Van_ R. N.

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Long-lasting effects of social stress on peritoneal inflammation in some strains of mice

Effects of macrophage depletion on peritoneal inflammation in swiss mice, edible frogs and goldfish