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1
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Glutathione conjugation in male reproductive system: Studies on glutathione-S-transferase of bull and boar epididymis.

100%
Ciszewska-Piłczyńska A., Barańczyk-Kuźma A.
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2000
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vol. 47
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issue 1
223-231
EN
Male reproductive organs are extremely sensitive to the negative influence of toxic environmental factors as well as drugs, and until now not many attempts have been made at studying the detoxication enzymes and the relationship between the activity of those enzymes and spermatozoa fertility. In the present work we studied cytosolic glutathione-S-transferases (GST, EC 2.5.1.18) from different parts (head, corpus and tail) of bull and boar epididymis. We isolated two molecular forms of GST from each part of epididymis, characterized their biochemical properties and examined the mechanism of the catalyzed reaction. On the basis of their substrate specificity and isoelectric point, the isoforms were found to belong to the near neutral GST class mi. All examined GST forms exhibited higher affinity towards GSH than towards 1-chloro-2,4-dinitrobenzene (CDNB) and bull epididymis GST forms showed biphasic Lineweaver-Burk double reciprocal curves in the presence of GSH as a variable substrate. Boar epididymis anionic GST had the -SH groups both in the GSH and the CDNB binding place, whereas the cationic GST form - arginine residues in the CDNB binding place. Bull epididymis GST forms contained neither thiol nor arginine residues essential for catalytic activity.
2
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Purification and some properties or human heart arginase

81%
Barańczyk-Kuźma A., Skrzypek-Osiecka I., Zalejska M., Porembska Z.
Acta Biochimica Polonica
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1980
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vol. 27
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issue 3-4
181-189
3
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Differences in glutathione S-transferase pi expression in transgenic mice with symptoms of neurodegeneration

81%
Kaźmierczak B., Kuźma-Kozakiewicz M., Usarek E., Barańczyk-Kuźma A.
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2011
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vol. 58
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issue 4
621-626
EN
Glutathione S-transferase pi (GST pi) is an enzyme involved in cell protection against toxic electrophiles and products of oxidative stress. GST pi expression was studied in transgenic mice hybrids (B6-C3H) with symptoms of neurodegeneration harboring SOD1G93A (SOD1/+), Dync1h1 (Cra1/+) and double (Cra1/SOD1) mutations, at presymptomatic and symptomatic stages (age 70, 140, 365 days) using RT-PCR and Western blotting. The main changes in GST pi expression were observed in mice with the SODG93A mutation. In SOD1/+ and Cra1/SOD1 transgenics, with the exception of cerebellum, the changes in GST pi-mRNA accompanied those in GST pi protein. In brain cortex of both groups the expression was unchanged at the presymptomatic (age 70 days) but was lower at the symptomatic stage (age 140 days) and at both stages in hippocampus and spinal cord of SOD1/+ but not of Cra1/SOD1 mice compared to age-matched wild-type controls. In cerebellum of the presymptomatic and the symptomatic SOD1/+ mice and presymptomatic Cra1/SOD1 mice, the GST pi-mRNA was drastically elevated but the protein level remained unchanged. In Cra1/+ transgenics there were no changes in GST pi expression in any CNS region both on the mRNA and on the protein level. It can be concluded that the SOD1G93A but not the Dync1h1 mutation significantly decreases detoxification efficiency of GST pi in CNS, however the Dync1h1 mutation reduces the effects caused by the SOD1G93A mutation. Despite similarities in neurological symptoms, the differences in GST pi expression between SOD1/+ and Cra1/+ transgenics indicate a distinct pathogenic entity of these two conditions.
4
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Arginase isoenzymes in human cirrhotic liver

81%
Chrzanowska A., Gajewska B., Barańczyk-Kuźma A.
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issue 3
465-469
EN
Cirrhosis leads to an inability of the liver to perform its biochemical functions. It can also lead to hepatocellular carcinoma in which, as we showed lately, arginase isoenzyme pattern changes. The present work presents our results on arginase isoenzymes and their possible role in liver cirrhosis. The study was performed on tissues obtained during liver transplantation from 60 patients with liver cirrhosis, and on samples of histologically normal liver (control) from 40 patients with benign or colorectal cancer liver metastases removed during surgery, 6-7 cm from the tumor border. Arginase isoenzymes AI (so-called liver-type arginase) and AII (called extrahepatic arginase) were identified by Western blotting and isolated by ion-exchange chromatography. Their expression on mRNA level was studied by RT-PCR. A significant decrease in arginase activity, dependent of the liver clinical stage, was observed in cirrhotic tissue. Arginase AI activity and its mRNA level were significantly decreased in cirrhotic liver, whereas the activity and expression of arginase AII were concurrently raised, as compared to normal liver. Since arginase AI is a key enzyme of the urea cycle, whereas arginase AII most probably takes part in the biosynthesis of ornithine and polyamines, the defective ammonia inactivation and increased collagen biosynthesis observed in cirrhotic liver may be related to the changes in arginase AI and AII levels, respectively.
5
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Sulfation of catecholamines, hypersensitive and hyposensitive drugs by monkey brain cortex enzymes

81%
Barańczyk-Kuźma A., Audus K. L., Borchardt R. T.
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vol. 40
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issue 1
189-192
6
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Phenol sulphotransferase: Purification and characterization of the rat kidney and stomach enzymes

81%
Barańczyk-Kuźma A., Borchardt R. T., Pinnick C. L.
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vol. 32
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issue 1
35-45
7
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The effect of exo- and endogenous compounds on the activity of glutathione-S-transferase from monkey brain

81%
Barańczyk-Kuźma A., Barszczewska I., Audus K. L.
Acta Biochimica Polonica
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1992
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vol. 39
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issue 1
133-138
8
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Changes in kinesin expression in the CNS of mice with dynein heavy chain 1 mutation

81%
Kuźma-Kozakiewicz M., Kaźmierczak B., Usarek E., Barańczyk-Kuźma A.
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2013
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vol. 60
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issue 1
51-55
EN
Dysfunction of fast axonal transport, vital for motor neurons, may lead to neurodegeneration. Anterograde transport is mediated by N-kinesins (KIFs), while retrograde transport by dynein 1 and, to a minor extent, by C-kinesins. In our earlier studies we observed changes in expression of N- and C-kinesins (KIF5A, 5C, C2) in G93ASOD1-linked mouse model of motor neuron degeneration. In the present work we analyze the profile of expression of the same kinesins in mice with a dynein 1 heavy chain mutation (Dync1h1, called Cra1), presenting similar clinical symptoms, and in Cra1/SOD1 mice with milder disease progression than SOD1 transgenics. We found significantly higher levels of mRNA for KIF5A and KIF5C but not the KIFC2 in the frontal cortex of symptomatic Cra1/+ mice (aged 365 days) compared to the wild-type controls. No changes in kinesin expression were found in the spinal cord of any age group and only mild changes in the hippocampus. The expression of kinesins in the cerebellum of the presymptomatic and symptomatic mice (aged 140 and 365 days, respectively) was much lower than in age-matched controls. In Cra1/SOD1 mice the changes in KIFs expression were similar or more severe than in the Cra1/+ groups, and they also appeared in the spinal cord. Thus, in mice with the Dync1h1 mutation, which impairs dynein 1-dependent retrograde transport, expression of kinesin mRNA is affected in various structures of the CNS and the changes are similar or milder than in mice with double Dync1h1/hSOD1G93A mutations.
9
Content available remote

Properties of phenol sulphotransferase from brain of the monkey Rhesus macaca

80%
Barańczyk-Kuźma A., Audus K. L., Borchardt R. T.
Acta Biochimica Polonica
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1992
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vol. 39
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issue 1
153-158
10
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Glutathione-S-transferase from boar testis: properties of the cytosolic and microsomal forms

80%
Ciszewska-Piłczyńska A., Barańczyk-Kuźma A.
Acta Biochimica Polonica
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1992
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vol. 39
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issue 1
139-145
11
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Placenta as a protecting barrier - some properties of glutathione-S-transferase

80%
Barszczewska I., Barańczyk-Kuźma A.
Acta Biochimica Polonica
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1992
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vol. 39
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issue 1
147-152
12
Content available remote

Sulfoconjugation of exo- and endogenous phenols: species and tissue specificity

79%
Barańczyk-Kuźma A.
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vol. 40
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issue 4
455-463
13
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Glutathione S-transferase pi as a target for tricyclic antidepressants in human brain.

71%
Barańczyk-Kuźma A., Kuźma M., Gutowicz M., Kaźmierczak B., Sawicki J.
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vol. 51
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issue 1
207-212
EN
GST pi, the main glutathione S-transferase isoform present in the human brain, was isolated from various regions of the brain and the in vitro effect of tricyclic antidepressants on its activity was studied. The results indicated that amitripyline and doxepin - derivatives of dibenzcycloheptadiene, as well as imipramine and clomipramine - derivatives of dibenzazepine, inhibit the activity of GST pi from frontal and parietal cortex, hippocampus and brain stem. All these tricyclics are noncompetitive inhibitors of the enzyme with respect to reduced glutathione and noncompetitive (amitripyline, doxepin) or uncompetitive (imipramine, clomipramine) with respect to the electrophilic substrate. Their inhibitory effect is reversible and it depends on the chemical structure of the tricyclic antidepressants rather than on the brain localization of the enzyme. We conclude that the interaction between GST pi and the drugs may reduce their availability in the brain and thus affect their therapeutic activity. On the other hand, tricyclic antidepressants may decrease the efficiency of the enzymatic barrier formed by GST and increase the exposure of brain to toxic electrophiles. Reactive electrophiles not inactivated by GST may contribute in adverse effects caused by these drugs.
14
Content available remote

Properties of phenol sulfotransferase from bovine brain

60%
Drobisz D., Barańczyk-Kuźma A.
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vol. 40
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issue 1
125-127
15
Content available remote

Heterogeneity of the bovine brain glutathione-S-transferase

60%
Barańczyk-Kuźma A., Drobisz D.
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vol. 40
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issue 1
100-102
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