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1

Laccase-mediated reactions ? mechanism and biotechnology applications

100%
Polak J., Jarosz-Wilkolazka A.
|
2007
|
issue 4
82-94
EN
Laccases catalyze oxidative conversion of a broad range of substrates such as phenols or lignin-derivatives. They also have an ability to induce oxidative coupling reactions of the free radical intermediates to related phenolic structures. The application of laccases-mediated coupling reactions in white biotechnology represents a promising alternative to chemical synthesis of products of commercial importance. The goal of this review was to present potential application of fungal laccases, focusing specifically on their use as biocatalysts in environmental friendly synthesis of new molecules.
2

Screening methods of industrial strain mutants

81%
Galas E., Kwapisz E., Polak J.
Biotechnologia
|
1995
|
issue 3
104-119
EN
The screening methods used for improvement of industrial strains are presented.Two general categories of methods have been distinguished: random screening and rational screening.Major techniques employed in rational screening are based on the selection of auxotropic mutants, reverants of non-producers, strain resistant to some factors like toxic analogues of metabolism intermediates, induction and catabolite regression or end-product inhibition, mutants with modified cell wall permeability and microorganisms tolerating somenutrients and precursors of secondary metabolites.
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