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Chlamydomonas reinhardtii - modelowy organizm w badaniach cyklu komórkowego, chloroplastowego i mitochondrialnego

100%

Matusiak-Mikulin K., Zielińska E., Tukaj Z.

Kosmos

2011

vol. 60

issue 1-2

103-112

Względna łatwość hodowli, krótki cykl komórkowy, znana sekwencja genomu, a także duża ilość różnego rodzaju mutantów czynią z Chlamydomonas reinhardtii atrakcyjny organizm modelowy w badaniach dotyczących rozwoju i funkcjonowania komórki. Struktura genomu jądrowego i chloroplastowego C. reinhardtii wykazuje więcej cech wspólnych z genomami roślin wyższych niż prostych eukariontów. Genom mitochondrialny tego organizmu jest jednym z najmniejszych dotychczas poznanych u roślin i występuje w postaci liniowej, dwuniciowej cząsteczki. Zsekwencjonowanie gnomów Chlamydomonas reinhardtii oraz możliwość ich względnie łatwej transformacji stwarza dogodne podstawy do badania molekularnych uwarunkowań przebiegu cyklu komórkowego i zależności pomiędzy cyklem komórkowym, chloroplastowym i mitochondrialnym.

The relative ease of culture, a short cell cycle, known whole genome sequence and a large number of different types of Chlamydomonas reinhardtii mutants, make this unicellular green alga a very attractive model organism for studies of the development and functioning of cell.The structure of nuclear and chloroplast genome of C. reinhardtii is more resembling genomes of vascularplants than simple eukaryotes. Mitochondrial genome of this organism is one of the smallest in plants and occurs as a linear, double-stranded molecule. Known whole genome sequence and the possibility of their transformation have allowed Chlamydomonas reinhardtii to become a highly valuable model for molecular approaches of cell cycle regulation and relationship between the cell, mitochondrial and chloroplast cycle.

The correlation analysis of WWOX expression and cancer related genes in neuroblastoma- a real time RT-PCR study

76%

Nowakowska M., Płuciennik E., Wujcicka W., Sitkiewicz A., Kazanowska B., Zielińska E., Bednarek A.

2014

vol. 61

issue 1

91-97

Neuroblastoma is one of the most common paediatric cancers, described as unpredictable due to diverse patterns of behaviour. WWOX is a tumour suppressor gene whose expression is reduced in many tumour types. Loss of its expression was shown to correlate with more aggressive disease stage and mortality rate. The aim of this study was to investigate the role of the WWOX tumour suppressor gene in neuroblastoma formation. We performed real-time RT-PCR to analyse levels of WWOX expression in 22 neuroblastic tumour samples in correlation with genes involved in cell cycle regulation (CCNE1, CCND1), proliferation (MKI67), apoptosis (BCL2, BIRC5, BAX) and signal transduction (EGFR, ERBB4). We also evaluated two potential mechanisms - promoter methylation (MethylScreen method) and loss of heterozygosity (LOH) status, which could be connected with regulation of WWOX gene expression. We found a positive correlation between WWOX gene and BCL2 and HER4 JM-a and negative with cyclin D1 and E1. Our observations are consistent with previous findings and emphasise the role of WWOX in cell cycle and apoptosis regulation. Moreover, strong positive association with HER4 JM-a in this tumour type may indicate a role for WWOX in neuroblastoma cell differentiation. The presented results indicate that LOH in locus D16S3096 (located in intron 8) may be involved in the regulation of WWOX mRNAexpression. However, no association between methylation status of WWOX promoter and its expression was observed.

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1 Acta Biochimica Polonica

1 Kosmos

1 2014

1 2011

2 Zielińska E.

1 Bednarek A.

1 Kazanowska B.

1 Matusiak-Mikulin K.

1 Nowakowska M.

1 Płuciennik E.

1 Sitkiewicz A.

1 Tukaj Z.

1 Wujcicka W.

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Chlamydomonas reinhardtii - modelowy organizm w badaniach cyklu komórkowego, chloroplastowego i mitochondrialnego

The correlation analysis of WWOX expression and cancer related genes in neuroblastoma- a real time RT-PCR study