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In vitro organogenesis induction in Sida hermaphrodita Rusby by cort

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Przyborowski J. A., Szczukowski S., Tworkowski J., Stolarski M.

Biotechnologia

2004

issue 2

131-136

Sida hermaphrodita Rusby belongs to the Malvaceae family. It is a perennial plant. Biomass obtained from sida plantations may constitute excellent raw material for renewable energy production. Sida hermaphrodita Rusby reproduces generatively, but its vegetative reproduction is also possible by root-cuttings, division of the underground part into particular roots, or cuttings obtained from green above-ground shoots. Sida seeds should be sown not earlier than after one year of storage, because immediately after harvest the percentage of germinated seeds is as low as 2%, after twelve months of storage 77.5%, to drop in successive months. Moreover, due to their hardness, sida seeds require special treatment. Therefore, an attempt was made at developing a method for in vitro micro-reproduction of sida plants, as an alternative method of seed material production. Two kinds of explants were used in the experiment, i.e. leaf discs 8 mm in diameter and 3 mm fragments of leaf stalks. The growth regulators were added to the MS medium in combinations, i.e.: IAA + kinetin; IAA + kinetin + BAP; TDZ + 2.4-D; 2ip + 2.4-D. The callus formed was crumbled and transferred to two kinds of medium: MS without growth regulators and MS with kinetin, NAA, GA3 and 40 g l-1 of sucrose. The most intensive growth of callus was observed on media containing 2ip and 2,4-D, but the highest number of roots and developing shoots was obtained on MS medium without fitohormones.

Intra- and interspecific pollination between Lupinus albus L., Lupinus mutabilis Sweet. and Lupinus angustifolius L.

100%

Przyborowski J. A., Samborska-Ciania A., Wiwart M.

Journal of Applied Genetics

1996

vol. 37

issue 3

261-275

The pollen grain germination and the growth of pollen tubes after intra and interspecific reciprocal polination of Lupinus albus L., Lupinus mutabilis Sweet. and Lupinus angustifolius L. were examined.Results of microscopic observations of pollen grain germination and pollen tube growth enable to deny the existence of certain barriers that prevent fertilization after pollination of L.albus flowers with pollen of L.angustifolius and vice versa, as well as after pollination of L.mutabilis flowers with pollen of L.angustifolius and vice versa.Some delay in pollen grain germination and a slower growth of pollen tubes were observed in all combinations, as related to intraspecific ones, though in case of each intrspecific combination pollen tubes reached the ovary.Moreover, a different level of pollination grain germination and pollen tube growth, depending on the direction of pollination, were found.The noted pollination efficiency may generally considered to be low.

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1 Biotechnologia

1 Journal of Applied Genetics

1 2004

1 1996

2 Przyborowski J. A.

1 Samborska-Ciania A.

1 Stolarski M.

1 Szczukowski S.

1 Tworkowski J.

1 Wiwart M.

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In vitro organogenesis induction in Sida hermaphrodita Rusby by cort

Intra- and interspecific pollination between Lupinus albus L., Lupinus mutabilis Sweet. and Lupinus angustifolius L.