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2013 | 85 | 7 | 387-394

Article title

Repair of the peripheral nerve gap with epineural sheath conduit to prevent muscle denervation atrophy in the diabetic rat model

Content

Title variants

Languages of publication

EN

Abstracts

EN
Muscle denervation atrophy is a result of lower motor neuron injury, thus an early restitution of muscle stimulation is essential in prevention of atrophic changes.The aim of the study was to evaluate the new application of naturally occurring epineural sheath conduit in repair of the peripheral nerve gap to prevent development of muscle denervation atrophy.Material and methods. We used the model of 20 mm sciatic nerve gap, resulting in denervation atrophy of the gastrocnemius muscle in the diabetic rats (DM type 2, n=42, Zucker Diabetic Fatty strain). We applied the epineural sheath conduit created from the autologous sciatic nerve for gap repair. Muscle atrophy was assessed with the Gastrocnemius Muscle Index (GMI) and microscopic muscle morphometry (mean fiber area) at 6 and 12 postoperative week. Muscle regeneration in the experimental group was compared to the gold-standard technique of autologous nerve grafting for the repair of created nerve gap.Results. The GMI evaluation revealed comparable muscle mass restoration in groups with nerve repair using both epineural sheath and standard autologous nerve grafting (reaching 28 and 35% of contralateral muscle mass at 12 postoperative week, respectively, p=0.1), and significantly better restoration when compared to the negative control group (no repair, 20%, p<0.01). Micromorphometry confirmed significantly larger area of the regenerated muscle fibers in groups with both nerve grafting and epineural sheath conduit repair (reaching for both ca. 42% of the non-operated side), when compared to severe atrophic outcome when no nerve repair was performed (14% of the control fiber area, p<0.0001). The effectiveness of epineural conduit technique in muscle mass restoration was observed between 6 and 12 weeks after nerve repair - when gastrocnemius muscle mass increased by 12%.Conclusions. Peripheral nerve gap repair with naturally occurring epineural sheath conduit is effective in prevention of muscle denervation atrophy. This method is applicable in diabetic model conditions, showing results of regeneration which are comparable to the autologous nerve graft repair

Publisher

Year

Volume

85

Issue

7

Pages

387-394

Physical description

Dates

published
1 - 07 - 2013
online
13 - 08 - 2013

Contributors

  • Department of General Surgery, Oncological Gastroenterology, and Plastic Surgery, Medical University in Poznan, Kierownik: prof. dr hab. M. Drews
  • Department of Plastic Surgery, Cleveland Clinic, Cleveland OH
  • 60-355 Poznań, ul. Przybyszewskiego 49
  • Department of Plastic Surgery, Cleveland Clinic, Cleveland OH
author
  • Department of Plastic Surgery, Cleveland Clinic, Cleveland OH
author
  • Department of Plastic Surgery, Cleveland Clinic, Cleveland OH
author
  • Department of General Surgery, Oncological Gastroenterology, and Plastic Surgery, Medical University in Poznan, Kierownik: prof. dr hab. M. Drews
  • Department of Plastic Surgery, Cleveland Clinic, Cleveland OH

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_pjs-2013-0059
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