SIL-based confocal fluorescence microscope for investigating individual nanostructures

• Authors: Bartosz Krajnik , Tim Schulte , Dawid Piątkowski , Nikodem Czechowski , Eckhard Hofmann , Sebastian Mackowski
• Languages of publication: EN

Abstracts

EN

We developed a fluorescence confocal microscope equipped with a hemispherical solid immersion lens (SIL) and apply it to study the optical properties of light-harvesting complexes. We demonstrate that the collection efficiency of the SIL-equipped microscope is significantly improved, as is the spatial resolution, which reaches 600 nm. This experimental setup is suitable for detailed studies of physical phenomena in hybrid nanostructures. In particular, we compare the results of fluorescence intensity measurements for a light-harvesting peridinin-chlorophyll-protein (PCP) complex with and without the SIL.

Keywords

EN

confocal microscopy; light-harvesting complex; solid immersion lens; fluorescence imaging

• Publisher: De Gruyter Open
• Journal: Open Physics
• Year: 2011
• Volume: 9
• Issue: 2
• Pages: 293-299

Dates

published

1 - 4 - 2011

online

20 - 2 - 2011

Contributors

author

Bartosz Krajnik

• Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, Torun, Poland

author

Tim Schulte

• Department of Biology and Biotechnology, Ruhr-University Bochum, Bochum, Germany

author

Dawid Piątkowski

• Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, Torun, Poland

author

Nikodem Czechowski

• Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, Torun, Poland

author

Eckhard Hofmann

• Department of Biology and Biotechnology, Ruhr-University Bochum, Bochum, Germany

author

Sebastian Mackowski

• Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, Torun, Poland

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Identifiers

DOI

10.2478/s11534-010-0098-5