Enzymatic oxidation of substituted tryptamines catalysed by monoamine oxidase

• Authors: Sylwia Dragulska , Marianna Kańska
• Languages of publication: EN

Abstracts

EN

The enzymatic deamination of 5-fl uorotryptamine and 5-hydroxytryptamine, 5-HT, catalysed by enzyme monoamine oxidase A (MAO-A, EC 1.4.3.4) was investigated using the kinetic (KIE) and solvent (SIE) isotope effects methods. The numerical values of deuterium isotope effects in the (1R) positions of 5-F-tryptamine were determined using non-competitive spectrophotomeric method. Isotopologue 5-F-[(1R)- -2H]-tryptamine, needed for kinetic studies was obtained by enzymatic decarboxylation of 5´-fl uoro-L-tryptophan, 5´-F-L-Trp, in fully deuteriated medium.

Keywords

EN

monoamine oxidase (MAO); tryptamine; serotonin; isotope effects

• Publisher: De Gruyter Open
• Journal: Nukleonika
• Year: 2014
• Volume: 59
• Issue: 3
• Pages: 91-95

Dates

published

1 - 8 - 2014

received

1 - 3 - 2013

online

12 - 9 - 2014

accepted

26 - 6 - 2014

Contributors

author

Sylwia Dragulska

• Department of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland, Tel.: [phone] ext. 509, Fax: [phone] ext. 434

author

Marianna Kańska

• Department of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland, Tel.: [phone] ext. 509, Fax: [phone] ext. 434

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Identifiers

DOI

10.2478/nuka-2014-0015