Determination of N-methyl-2-pyrrolidone and its metabolites in urine by micellar electrokinetic chromatography

• Authors: Jana Lokajová , Simo Porras , Eivor Elovaara , Susanne Wiedmer
• Languages of publication: EN

Abstracts

EN

A fast and accurate micellar electrokinetic capillary chromatography (MEKC) method was developed for monitoring N-methyl-2-pyrrolidone (NMP) exposure. Baseline separation of NMP and its main metabolites: 5-hydroxy-N-methyl-2-pyrrolidone (5HNMP), N-methylsuccinimide (MSI), 2-hydroxy-N-methylsuccinimide (2HMSI), and 2-pyrrolidone (2P) was obtained within 6 min in an uncoated fused silica capillary using 5 mM phosphate buffer and 140 mM sodium dodecyl sulfate (pH 7.1) as background electrolyte (BGE). On-line UV-detection was performed at 200 nm and the applied electric field was 400 V cm−1. Possible interference of BGE-induced system peaks on separation was investigated by computer simulation and no such interference was observed. The developed MEKC method combined with solid phase extraction for sample preparation was successfully applied to the analysis of urine of rats exposed to NMP. The urinary excretion was determined in 0–6 h and 6–24 h specimens collected after an intragastic administration of 308 mg NMP / kg rat body weight. The results of NMP disposition kinetics in rat urine are reported for NMP and metabolites. [...]

Keywords

EN

NMP; Urinary metabolites; MEKC; Method development; System peaks

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2011
• Volume: 9
• Issue: 5
• Pages: 825-833

Dates

published

1 - 10 - 2011

online

24 - 7 - 2011

Contributors

author

Jana Lokajová

author

Simo Porras

• Finnish Institute of Occupational Health, FI-00250, Helsinki, Finland

author

Eivor Elovaara

• the Finnish Institute of Occupational Health, Helsinki, Finland

author

Susanne Wiedmer

• Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, FI-00014, Helsinki, Finland

References

• [1] ACGIH, N-Methyl-2-pyrrolidone BEI®, 2010 TLVs® and BEIs® with 7th Edition Documentation (The American Conference of Governmental Industrial Hygienists, Cincinnati, Ohio, 2010)
• [2] IPCS, Concise International Chemical Assessment Document No. 35, N-Methyl-2-Pyrrolidone. Inter-Organization Programme for the Sound Management of Chemicals (IOMC) (World Health Organization, Geneva, 2001)
• [3] SCOEL, Recommendation of the Scientific Committee on Occupational Exposure Limits for N-methylpyrrolidone (Scientific Committee on Occupational Exposure Limits, Brussels, 2007)
• [4] B. Åkesson, Arbete Hälsa 40, 1 (1994)
• [5] Ex-European Chemicals Bureau (Ex-ECB), Institute for Health and Consumer Protection (IHCP), European Commission’s Joint Research Centre (JRC). http://ecb.jrc.ec.europa.eu/
• [6] Y. Marcus, The Properties of Solvents (Wiley, Chichester, 1998)
• [7] B. Åkesson, B.A.G. Jönsson, Drug Metab. Dispos. 25, 267 (1997)
• [8] M.A. Carnerup, A.M. Saillenfait, B.A.G. Jönsson, Food Chem. Toxicol. 43, 1441 (2005) http://dx.doi.org/10.1016/j.fct.2005.04.007[Crossref]
• [9] M.A. Carnerup, M. Spanne, B.A.G. Jönsson, Toxicol. Lett. 162, 139 (2006) http://dx.doi.org/10.1016/j.toxlet.2005.09.035[Crossref]
• [10] K. Sitarek, A. Kilanowicz, Int. J. Occup. Med. Environ. Health 19, 142 (2006) http://dx.doi.org/10.2478/v10001-006-0018-5[Crossref]
• [11] P. Akrill, J. Cocker, S. Dixon, Toxicol. Lett. 134, 265 (2002) http://dx.doi.org/10.1016/S0378-4274(02)00175-3[Crossref]
• [12] B.A.G. Jönsson, B. Åkesson, J. Chromatogr. B 704, 151 (1997) http://dx.doi.org/10.1016/S0378-4347(97)00452-0[Crossref]
• [13] B.A.G. Jönsson, B. Åkesson, Chromatographia 46, 141 (1997) http://dx.doi.org/10.1007/BF02495324[Crossref]
• [14] B.A.G. Jönsson, B. Åkesson, J. Chromatogr. B 694, 351 (1997) http://dx.doi.org/10.1016/S0378-4347(97)00125-4[Crossref]
• [15] B.A.G. Jönsson, B. Åkesson, Int. Arch. Occup. Environ. Health 74, 289 (2001) http://dx.doi.org/10.1007/PL00007946[Crossref]
• [16] B.A.G. Jönsson, B. Åkesson, Int. Arch. Occup. Environ. Health 76, 267 (2003)
• [17] S.A. Keener, R. Wrbitzky, M. Bader, Int. Arch. Occup. Environ. Health 80, 327 (2007) http://dx.doi.org/10.1007/s00420-006-0138-z[Crossref]
• [18] D. Ligocka, D. Lison, V. Haufroid, J. Chromatogr. B 778, 223 (2002) http://dx.doi.org/10.1016/S0378-4347(01)00441-8[Crossref]
• [19] B. Åkesson, B.A.G. Jönsson, Scand. J. Work Environ. Health 26, 213 (2000) [Crossref]
• [20] M.A. Carnerup, B. Åkesson, B.A.G. Jönsson, J. Chromatogr. B 761, 107 (2001) http://dx.doi.org/10.1016/S0378-4347(01)00312-7[Crossref]
• [21] J.P. Payan, D. Beydon, J.P. Fabry, I. Boudry, B. Cossec, E. Ferrari, Drug Metab. Dispos. 30, 1418 (2002) http://dx.doi.org/10.1124/dmd.30.12.1418[Crossref]
• [22] J.P. Payan, I. Boudry, D. Beydon, J.P. Fabry, M.C. Grandclaude, E. Ferrari, J.C. Andre, Drug Metab. Dispos. 31, 659 (2003) http://dx.doi.org/10.1124/dmd.31.5.659[Crossref]
• [23] B. Åkesson, M.A. Carnerup, B.A.G. Jönsson, Scand. J. Work Environ. Health 30, 306 (2004) [Crossref]
• [24] R.J. Geise, N.I. Machnicki, R.M. Ianniello, J. Liq. Chromatogr. 16, 3699 (1993) http://dx.doi.org/10.1080/10826079308019662[Crossref]
• [25] F. Foret, L. Křivánková, P. Boček, Capillary Zone Electrophoresis (VCH, Weinheim, 1993)
• [26] S. Terabe, In: J.P. Landers (Ed.), Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques (CRC Press, London, 2008)
• [27] J.L. Beckers, P. Gebauer, P. Boček, Electrophoresis 22, 3648 (2001) http://dx.doi.org/10.1002/1522-2683(200109)22:17<3648::AID-ELPS3648>3.0.CO;2-Q[Crossref]
• [28] B. Gaš, V. Hruška, M. Dittmann, F. Bek, K. Witt, J. Sep. Sci. 30, 1435 (2007) http://dx.doi.org/10.1002/jssc.200600502[Crossref]
• [29] B. Gaš, E. Kenndler, Electrophoresis 25, 3901 (2004) http://dx.doi.org/10.1002/elps.200406159[Crossref]
• [30] P. Gebauer, J.L. Beckers, P. Boček, Electrophoresis 23, 1779 (2002) http://dx.doi.org/10.1002/1522-2683(200206)23:12<1779::AID-ELPS1779>3.0.CO;2-G[Crossref]
• [31] J.N. Miller, J.C. Miller, Statistics and Chemometrics for Analytical Chemistry, 5th edition (Pearson, Harlow, 2005)
• [32] J. Inczédy, T. Lengyel, A.M. Ure, IUPAC Compendium on Analytical Nomenclature, Definitive rules 1997 (International Union of Pure and Applied Chemistry, 1997)
• [33] D.G. McLaren, O. Boulat, D.D.Y. Chen, Electrophoresis 23, 1912 (2002) http://dx.doi.org/10.1002/1522-2683(200206)23:12<1912::AID-ELPS1912>3.0.CO;2-L[Crossref]
• [34] E. Kenndler, J. Microcolumn Sep. 10, 273 (1998) http://dx.doi.org/10.1002/(SICI)1520-667X(1998)10:3<273::AID-MCS6>3.0.CO;2-D[Crossref]
• [35] B. Gaš, P. Coufal, M. Jaroš, J. Muzikář, I. JelÍnek, J. Chromatogr. A 905, 269 (2001) http://dx.doi.org/10.1016/S0021-9673(00)00983-3[Crossref]
• [36] P. Gebauer, P. Pantůčková, P. Boček, J. Chromatogr. A 894, 89 (2000) http://dx.doi.org/10.1016/S0021-9673(00)00707-X[Crossref]
• [37] J.L. Beckers, P. Boček, Electrophoresis 23, 1947 (2002) http://dx.doi.org/10.1002/1522-2683(200206)23:12<1947::AID-ELPS1947>3.0.CO;2-M[Crossref]
• [38] J. Lokajová, V. Hruška, E. Tesařová, B. Gaš, Electrophoresis 29, 1189 (2008) http://dx.doi.org/10.1002/elps.200700780[Crossref]
• [39] H. Anundi, S. Langworth, G. Johanson, M.L. Lind, B. Åkesson, L. Friis, N. Itkes, E. Söderman, B.A.G. Jönsson, C. Edling, Int. Arch. Occup. Environ. Health 73, 561 (2000) http://dx.doi.org/10.1007/s004200000157[Crossref]
• [40] M. Bader, W. Rosenberger, T. Rebe, S.A. Keener, T.H. Brock, H.-J. Hemmerling, R. Wrbitzky, Int. Arch. Occup. Environ. Health 79, 357 (2006) http://dx.doi.org/10.1007/s00420-005-0065-4[Crossref]
• [41] A.S. Cohen, B.A.G. Jönsson, Chromatographia 65, 407 (2007) http://dx.doi.org/10.1365/s10337-007-0173-2[Crossref]
• [42] R. Kubota, Y. Endo, A. Takeuchi, Y. Inoue, H. Ogata, M. Ogawa, T. Nakagawa, N. Onda, G. Endo, J. Chromatogr. B 854, 204 (2007) http://dx.doi.org/10.1016/j.jchromb.2007.04.022[Crossref]
• [43] Y. Suzuki, Y. Endo, M. Ogawa, S. Yamamoto, A. Takeuchi, T. Nakagawa, N. Onda, J. Chromatogr. B 877, 3743 (2009) http://dx.doi.org/10.1016/j.jchromb.2009.08.036[Crossref]
• [44] DFG, List of MAK and BAT Values 2009, Maximum Concentrations and Biological Tolerance Values at the Work Place, Deutsche Forschungsgemeinschaft (Wiley-VCH, Weinheim, 2009)

Identifiers

DOI

10.2478/s11532-011-0062-2