On chromium direct ETAAS determination in serum and urine

• Authors: Nadica Todorovska , Irina Karadjova , Sonja Arpadjan , Trajce Stafilov
• Languages of publication: EN

Abstracts

EN

A simple, accurate and reliable method for direct electrothermal atomic absorption spectrometric (ETAAS) determination of chromium in serum and urine samples without any preliminary sample pretreatment is described. Instrumental parameters are optimized in order to define: the most suitable atomizer, optimal temperature program and efficient modifier. An appropriate quantification method is proposed taking into account a matrix interference study. Pyrocoated graphite tubes and wall atomization, pretreatment temperature of 700 °C, atomization temperature of 2600 °C, hydrogen peroxide as modifier and standard addition calibration are recommended. The accuracy of the method proposed for Cr determination in serum and urine was confirmed by comparative analysis of parallel samples after wet or dry ashing as well as by the analysis of two certified reference materials: Serum, Clin Rep 1 and Lypochek Urine, level 1. The detection and determination limits achieved for both matrices are 0.08 µg/L and 0.15 µg/L respectively. The relative standard deviation varied between 15 and 18 % for the chromium content in the samples in the range 0.08–0.2 µg/L and between 4 and 7 % for the chromium content in the range 0.2–2.0 µg/L for both matrices. [...]

Keywords

EN

Chromium; electrothermal atomic absorption spectrometry; serum; urine

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2007
• Volume: 5
• Issue: 1
• Pages: 230-238

Dates

published

1 - 3 - 2007

online

1 - 3 - 2007

Contributors

author

Nadica Todorovska

• Institute of Preventive Medical Care and Toxicology, Military Health Institution Center, 1000, Skopje, Republic of Macedonia

author

Irina Karadjova

• Faculty of Chemistry, University of Sofia, 1126, Sofia, Bulgaria

author

Sonja Arpadjan

• Faculty of Chemistry, University of Sofia, 1126, Sofia, Bulgaria

author

Trajce Stafilov

• Institute of Chemistry, Faculty of Science, St. Cyril and Methodius University, 1001, Skopje, Republic of Macedonia

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Identifiers

DOI

10.2478/s11532-006-0055-8