Oligonucleotides bearing pentaerythritol-derived mass tags

• Authors: Anu Kiviniemi , Petri Heinonen , Harri Lönnberg
• Languages of publication: EN

Abstracts

EN

Five different di-O-alkylated pentaerythritol phosphoramidite building blocks (2a–e) were synthesized and introduced into oligonucleotides to obtain mass-tagged probes (6a–f) useful in RNA transcription profiling. These non-nucleosidic mass tags allow categorization of oligonucleotide probes having identical nucleoside content and, hence, identification of the probe hybridized to RNA by mass spectrometry analysis. Hybridization properties of the oligonucleotide conjugates were elucidated by melting temperature measurements.

Keywords

EN

Oligonucleotide conjugate; pentaerythritol; mass tag; hybridization; phosphoramidite; ether

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2007
• Volume: 5
• Issue: 1
• Pages: 191-200

Dates

published

1 - 3 - 2007

online

1 - 3 - 2007

Contributors

author

Anu Kiviniemi

• Department of Chemistry, University of Turku, FIN-20014, Turku, Finland

author

Petri Heinonen

• Department of Chemistry, University of Turku, FIN-20014, Turku, Finland

author

Harri Lönnberg

• Department of Chemistry, University of Turku, FIN-20014, Turku, Finland

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Identifiers

DOI

10.2478/s11532-006-0046-9