Determination of aristolochic acid by capillary zone electrophoresis

• Authors: František Kvasnička , Rudolf Ševčík , Michal Voldřich , Jana Krátká
• Languages of publication: EN

Abstracts

EN

A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC.

Keywords

EN

aristolochic acid; food supplement; herbs; slimming; capillary zone electrophoresis

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2004
• Volume: 2
• Issue: 3
• Pages: 417-424

Dates

published

1 - 9 - 2004

online

1 - 9 - 2004

Contributors

author

František Kvasnička

• Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic

author

Rudolf Ševčík

• Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic

author

Michal Voldřich

• Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic

author

Jana Krátká

• Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic

References

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Identifiers

DOI

10.2478/BF02476197