THROMBORESISTANT SILICON PLATES MODIFIED WITH CHITOSAN AND HEPARIN BY THE LAYER-BY-LAYER ASSEMBLY METHOD

• Authors: Natalia N. Drozd , Balzhima Ts. Shagdarova , Yulia V. Zhuikova , Alla V. Il’ina , Michael N. Vasiliev , Tatiana M. Vasilieva , Aung Miat Hein , Valery P. Varlamov
• Languages of publication: EN

Abstracts

EN

Fifteen samples of silicone plates (PlateSi, area=12 cm2), with surfaces modified layer-by-layer with chitosan and unfractionated heparin, were obtained. The sample surfaces were pre-treated by cold oxygen plasma in a planar-type plasma chemical reactor with 50 W power before coating with layered polysaccharides. Pre-treatment was carried out in two alternative operation modes of the reactor, namely in the plasma etching mode and in the reactive-ion etching mode. Thromboresistance was assessed in vitro in contact with human blood. The thromboresistant silicon plates, modified layer-by-layer (3, 5, 7, and 9 bilayers) with chitosan, with molecular weights of 65 kDa, increased with the increase in the number of layers, up to 5. An increase in the duration of thromboresistance was observed in layer-by-layer modification of the surface of the plates with chitosan with a molecular weight of 200 kDa or with quaternized chitosan with a molecular weight of 200 kDa. Some samples of highly thromboresistant, modified PlateSi contributed to the adhesion of platelets and the haemolysis of red blood cells to a lesser extent than untreated silicon plates. The three most promising samples of modified PlateSi were selected.

Keywords

EN

AFM; chitosan; cold plasma treatment; heparin; layer-by-layer; polyelectrolyte; silicone rubber; thromboresistance

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2019
• Volume: 24
• Pages: 5 - 22

Contributors

author

Natalia N. Drozd

• National Research Centre for Haematology of the Ministry of Healthcare of the Russian Federation

author

Balzhima Ts. Shagdarova

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences

author

Yulia V. Zhuikova

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences

author

Alla V. Il’ina

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences

author

Michael N. Vasiliev

• Moscow Institute of Physics and Technology

author

Tatiana M. Vasilieva

• Moscow Institute of Physics and Technology

author

Aung Miat Hein

• Moscow Institute of Physics and Technology

author

Valery P. Varlamov

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences

References

• [1] Ong CS, Zhou X, Huang CY, Fukunishi T, Zhang H, Hibino N; (2017) Tissue engineered vascular grafts: current state of the field. Expert Rev Med Devices 14(5), 383-392. DOI:10.1080/17434440.2017.1324293.
• [2] Reis LA, Chiu LL, Feric N, Fu L, Radisic M; (2016) Biomaterials in myocardial tissue engineering. J Tissue Eng Regen Med 10(1), 11-28. DOI: 10.1002/term.1944.
• [3] Li ZK, Wu ZS, Lu T, Yuan HY, Tang H, Tang ZJ, Tan L, Wang B, Yan SM; (2016) Materials and surface modification for tissue engineered vascular scaffolds. J Biomater Sci Polym Ed 27 (15) 1534-1552. DOI: 10.1080/09205063.2016.1217607
• [4] Boire TC, Balikov DA, Lee Y, Guth CM, Cheung-Flynn J, Sung HJ; (2016) Biomaterial-Based Approaches to Address Vein Graft and Hemodialysis Access Failures. Macromol Rapid Commun 37(23), 1860-1880. DOI: 10.1002/marc.201600412.
• [5] Williams DF; (2012) Concepts in biocompatibility: New biomaterials, new paradigms and new testing regimes. In: Boutrand, J.-P. (ed), Biocompatibility and Performance of Medical Devices, Woodhead Publising: Philadelphia, NJ, USA, eBook ISBN: 9780857096456, Hardcover ISBN: 9780857090706, 3-17.
• [6] Zhao H, Zhang F, Liang G, Ye L, Zhang H, Niu L, Cheng L, Zhang M; (2016) Preparation and experimental research into retrievable rapamycin- and heparin-coated vena cava filters: a pilot study. J Thromb Thrombolysis 41(3), 422-432. DOI: 10.1007/s11239-015-1278-3.
• [7] Gostev AA, Laktionov PP, Karpenko AA; (2018) Modern polyurethanes in cardiovascular surgery. Angiol Sosud Khir 24(1), 29-38. PMID:29688192
• [8] Seckold T, Walker S, Dwyer T; (2015) A comparison of silicone and polyurethane PICC lines and postinsertion complication rates: a systematic review. J Vasc Access 16(3), 167-177. DOI: 10.5301/jva.5000330.
• [9] Stotesbury T, Illes M, Wilson P, Vreugdenhil AJ; (2017) The application of silicon sol-gel technology to forensic blood substitute development: Mimicking aspects of whole human blood rheology. Forensic Sci Int 270, 12-19. DOI: 10.1016/j.forsciint.2016.11.012.
• [10] Kono K, Shintani A, Okada H, Terada T; (2013) Preoperative simulations of endovascular treatment for a cerebral aneurysm using a patient-specific vascular silicone model. Neurol Med Chir (Tokyo) 53(5), 347–351. PMID: 23708228
• [11] Alves P, Cardoso R, Correia TR, Antunes BP, Correia IJ, Ferreira P; (2014) Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves. Colloids Surf B Biointerfaces 113, 25-32. DOI: 10.1016/j.colsurfb.2013.08.039.
• [12] Taniguchi T, Kyung K-H, Shiratori S; (2015) Layer-by-layer self-assembled thin films of chitin fibers and heparin with anti-thrombus characteristics. RSC Advances, 5(130), 107488–107496. DOI:10.1039/c5ra01853f
• [13] Hartmann H, Krastev R; (2017) Biofunctionalization of surfaces using polyelectrolyte multilayers. BioNanoMaterials, 18(1-2), article number 20160015. DOI:10.1515/bnm-2016-0015
• [14] Govindharajulu JP, Chen X, Li Y, Rodriguez-Cabello JC, Battacharya M, Aparicio C; (2017) Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants. Int J Mol Sci 18(2), article number 369. DOI: 10.3390/ijms18020369.
• [15] Follmann HDM, Naves AF, Martins AF, Félix O, Decher G, Muniz EC, Silva R; (2016) Advanced fibroblast proliferation inhibition for biocompatible coating by electrostatic layer-by-layer assemblies of heparin and chitosan derivatives. J Colloid Interf Sci 474, 9–17. DOI: 10.1016/j.jcis.2016.04.008
• [16] Boddohi S, Killingsworth CE, Kipper MJ; (2008) Polyelectrolyte Multilayer Assembly as a Function of pH and Ionic Strength Using the Polysaccharides Chitosan and Heparin. Biomacromolecules 9(7), 2021-2028. DOI: 10.1021/bm8002573
• [17] Sorlier P, Denuzie`re A, Viton C, Domard A; (2001) Relation between the degree of acetylation and the electrostatic properties of chitin and chitosan. Biomacromolecules 2(3), 765-772. DOI: 10.1021/bm015531+
• [18] van Veen JJ, Maclean RM, Hampton KK, Laidlaw S, Kitchen S, Toth P, Makris M; (2011) Protamine reversal of low molecular weight heparin: clinically effective? Blood Coagul Fibrinolysis 22(7), 565–570. DOI: 10.1097/MBC.0b013e3283494b3c
• [19] Liang Y, Kiick KL; (2014) Heparin-functionalized polymeric biomaterials in tissue engineering and drug delivery applications. Acta Biomater 10(4), 1588-1600. DOI: 10.1016/j.actbio.2013.07.031.
• [20] Owens DK, Wendt RC; (1969) Estimation of the surface free energy of polymers. J Appl Polym Sci 13(8), 1741-1747. DOI: 10.1002/app.1969.070130815
• [21] Shagdarova BTs, Ilyina AV, Lopatin SA, Kartashov MI, Arslanova LR, Dzhavakhiya VG, Varlamov VP; (2018) Study of the Protective Activity of Chitosan Hydrolyzate Against Septoria Leaf Blotch of Wheat and Brown Spot of Tobacco. Applied Biochem Microbiol, 54 (1), 71–75. DOI: 10.1134/S0003683818010118
• [22] Shagdarova B, Lunkov A, Il'ina A, Varlamov V; (2019) Investigation of the properties of N-[(2-hydroxy-3-trimethylammonium) propyl] chloride chitosan derivatives. Int J Biol Macromol. https://doi.org/10.1016/j.ijbiomac.2018.11.209
• [23] Wang X, Hu L, Li C, Gan L, He M, He X, Tian W, Li M, Xu L, Li Y , Chen Y; (2016) Improvement in physical and biological properties of chitosan/soy protein films by surface grafted heparin. Int J Biol Macromol 83:19-29. DOI: 10.1016/j.ijbiomac.2015.11.052.
• [24] Stuart R, Michel A; (1971) Monitoring heparin therapy with the activated partial thromboplastin time. Can Med Association J 104 (5), 385-388 PMCID: PMC1930883
• [25] Dash B, Rethore G, Monaghan M, Fitzgerald K, Gallagher W, Pandit A; (2010) The influence of size and charge of chitosan/polyglutamic acid hollow spheres on cellular internalization, viability and blood compatibility. Biomaterials 31 (32), 8188 – 8197. DOI: 10.1016/j.biomaterials.2010.07.067
• [26] Kolodziejczyk-Czepas J, Sieradzka M, Wachowicz B, Nowak P, Oleszek W, Stochmal A; (2016) The anti-adhesive and anti-aggregatory effects of phenolics from Trifolium species in vitro. Mol Cell Biochem 412:155–164 DOI: 10.1007/s11010-015-2620-y
• [27] Walkowiak B, Michalak E, Koziolkiewicz W, Cierniewski CS; (1989) Rapid photometric method for estimation of platelet count in blood plasma or platelet suspension. Thromb Res 56(6), 763-766 PMID:2633404
• [28] De Geyter N, Morent R; (2012) In: Ghista DN (ed) Biomedical science, engineering and technology, InTech, Rijeka, Croatia
• [29] Slepička P, Slepičková Kasálková N, Stránská E, Švorčík V; (2013) Surface Characterization of Plasma Treated Polymers for Applications as Biocompatible Carriers. Express Polymer Letters 7(6): 535-545. DOI: 10.3144/expresspolymlett.2013.50
• [30] Li M, Wu H, Wang Y, Yin T, Gregersen H, Zhang X, Liao X, Wang G; (2017) Immobilization of heparin/poly-l-lysine microspheres on medical grade high nitrogen nickel-free austenitic stainless steel surface to improve the biocompatibility and suppress thrombosis.
• [31] Mater SciEng C Mater Biol Appl. 73, 198-205. DOI: 10.1016/j.msec.2016.12.070

• Document Type: article