Oxidative stress and enzymatic antioxidant status of blood and synovial fluid in rheumatoid arthritis patients

• Authors: Alina Ostałowska , Bogdan Koczy , Ludmiła Słowińska , Aleksandra Kasperczyk , Michał Dobrakowski , Urszula Błaszczyk

Title variants

PL

Stres oksydacyjny i enzymatyczny układ entyoksydacyjny we krwi i płynie stawowym pacjentów z reumatoidalnym zapaleniem stawów

• Languages of publication: EN

Abstracts

EN

INTRODUCTION: Although the exact etiology of rheumatoid arthritis (RA) remains unknown, there is increasing evidence that reactive oxygen species (ROS) and oxidant/antioxidant imbalance are an important part of the pathogenesis of joint tissue injury. MATERIAL AND METHODS: The activities of: manganese superoxide dismutase (MnSOD) and copper-zinc superoxide dismutase (CuZnSOD) isoenzymes, catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST), and malondialdehyde (MDA) levels were determined in blood and synovial fluid samples from 178 RA patients and from 27 healthy controls. RESULTS: The RA patients showed increased antioxidant enzyme activities and MDA levels. Decreased synovial fluid viscosity was associated with a tendency for a changed antioxidant system with increased antioxidant enzyme activities, thereby suggesting a possible adaptation to ROS production in the blood and synovial fluid in RA patients. CONCLUSIONS: Correlating antioxidant enzyme activities and MDA levels to disease activity might provide further information about oxidative stress in RA pathogenesis.

PL

WSTĘP: W patogenezie reumatoidalnego zapalenia stawów jednym z mechanizmów uszkadzających struktury stawu są reakcje z udziałem reaktywnych form tlenu (RFT). MATERIAŁ I METODY: U 178 pacjentów z RZS oraz 27 zdrowych ochotników oznaczono we krwi i płynie stawowym aktywność enzymów antyoksydacyjnych: izoenzymów dysmutazy ponadtlenkowej manganowej (MnSOD) i cynkowo-miedziowej (CuZnSOD), katalazy (CAT), peroksydazy glutationowej (GPX), reduktazy glutationowej (GR) i transferazy-S-glutationowej (GST) oraz stężenie dialdehydu malonowego (MDA). WYNIKI: We krwi i płynie stawowym pacjentów z RZS dochodzi do pobudzenia układu antyoksydacyjnego ze wzrostem aktywności enzymów antyoksydacyjnych, zwiększonym stężeniem MDA oraz spadkiem lepkości płynu stawowego. WNIOSKI: Zależność między nasileniem zmian w układzie antyoksydacyjnym a aktywnością zapalenia stawów potwierdza udział RFT w patogenezie i przebiegu RZS.

Keywords

EN

antioxidant enzymes; enzymy antyoksydacyjne; płyn stawowy; reumatoidalne zapalenie stawów; rheumatoid arthritis; synovial fluid

Discipline

• MEDICINE: MEDICINE

• Publisher: Śląski Uniwersytet Medyczny w Katowicach
• Journal: Annales Academiae Medicae Silesiensis
• Year: 2016
• Volume: 70
• Pages: 196–205

Contributors

author

Alina Ostałowska

• MD, PhD, Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, ul. Jordana 19, 41-808 Zabrze, tel. [phone]

author

Bogdan Koczy

• Department of Orthopaedics, District Orthopaedic Hospital, Bytom

author

Ludmiła Słowińska

• Department of Biophysics, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice

author

Aleksandra Kasperczyk

• Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice

author

Michał Dobrakowski

• Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice

author

Urszula Błaszczyk

• Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice

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• Document Type: article