Zinc determination in extracts from bacopa monnieri shoot cultures into simulated gastric fluids.

• Authors: Bożena MUSZYŃSKA , Jacek ROJOWSKI , Maciej ŁOJEWSKI , Karolina DZIWULSKA , Włodzimierz OPOKA

Title variants

PL

Oznaczenie cynku w ekstraktach biomasy z kultur pędowych Bacopa monnieri do sztucznych soków trawiennych.

• Languages of publication: PL EN

Abstracts

EN

Bacopa monnieri (Water hyssop) is a hydrophyte occurring in Asia and the southern US states. This plant is known in India as Brahmi and has been used in Ayurvedic medicine for 5,000 years as sedative, supporting the treatment of insomnia and improving cerebral circulation. For the biological effect in this species are responsible triterpenoids saponins – bacosides. The aim of the study was to obtain the biomass of B. monnieri shoot in vitro cultures, and then evaluating the biomass growth and content of zinc. B. monnieri derived from in vitro culture on Murashige-Skoog medium (MS) and on the same medium, but enriched in organic additives and elements may be a good source of Zinc for the human body. The experiment was also conducted on the commercial formulations containing B. monnieri. For the determination of zinc ions DP ASV method was used. It was found that the use of highly mineralized water (mineral water “Stefan” – Spa Szczawnica) for extraction of B. monnieri biomass from in vitro culture provided the highest concentration of zinc in the material and for the biomass from MS medium with the addition of As (163.52, and 167.23 mg/mL, respectively). The lowest content was found in B. monnieri biomass from MS medium supplemented with serine, and the capsules product (13.54 and 14.57 mg/mL). For extracts of B. monnieri biomass from MS medium enriched with Zn salts into digestive juices the highest concentration of zinc was determined in gastric juice (6.88 mg/mL).

Keywords

EN

"Bacopa monnieri"; "DP-ASV method"; "in vitro culture"; "zinc"

Discipline

• BOTANY: BOTANY

• Publisher: ZOZ Ośrodek UMEA SHINODA-KURACEJO
• Journal: Medicina Internacia Revuo
• Year: 2015
• Volume: 26
• Issue: 105
• Pages: 175-179

Contributors

author

Bożena MUSZYŃSKA

• Department of Pharmaceutical Botany, Jagiellonian University Collegium Medicum, Medyczna 9, 30-688 Kraków, Poland

author

Jacek ROJOWSKI

• Department of Inorganic and Analytical Chemistry, Jagiellonian University Collegium Medicum, Medyczna 9, 30-688 Kraków, Poland

author

Maciej ŁOJEWSKI

• Department of Pharmaceutical Botany, Jagiellonian University Collegium Medicum, Medyczna 9, 30-688 Kraków, Poland

author

Karolina DZIWULSKA

• Department of Inorganic and Analytical Chemistry, Jagiellonian University Collegium Medicum, Medyczna 9, 30-688 Kraków, Poland

author

Włodzimierz OPOKA

• Department of Inorganic and Analytical Chemistry, Jagiellonian University Collegium Medicum, Medyczna 9, 30-688 Kraków, Poland

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• Document Type: article