ISOLATION, MOLECULAR CLONING AND CHARACTERISATION OF TWO GENES CODING CHITIN DEACETYLASE FROM MUCOR CIRCINELLOIDES IBT-83

• Authors: Michał Kaczmarek , Katarzyna Struszczyk-Świta , Tomasz Florczak , Mirosława Szczęsna-Antczak , Tadeusz Antczak
• Languages of publication: EN

Abstracts

EN

Chitosan is a linear N-deacetylated derivative of chitin, soluble in acetic solutions. The deacetylation of chitin can be achieved enzymatically using chitin deacetylase (ChDa) (EC 3.5.1.41), which hydrolyses the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin and chitosan. Complementary DNA (cDNA), which encodes ChDa, was isolated from M. rouxii s well as other fungi. Chitin deacetylase activity was detected in partially purified and concentrated crude extract of the protein from Mucor circinelloides IBT-83. Additionally, two open reading frames (ORF), putatively encoding ChDa, were identified and amplified from cDNA of this strain. Each ORF was molecularly cloned and sequenced. Amino acid sequences of ChDaI and ChDaII were predicted, using nucleotide sequences of these cDNA clones, and analysed by means of bioinformatics tools.

Keywords

EN

chitin deacetylase; genes; molecular cloning; sequences analysis

• Publisher: Polish Chitin Society
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2016
• Volume: 21
• Pages: 93-103

Contributors

author

Michał Kaczmarek

• Institute of Technical Biochemistry, Lodz University of Technology ul. Stefanowskiego 4/10, 90-924 Łódź

author

Katarzyna Struszczyk-Świta

• Institute of Technical Biochemistry, Lodz University of Technology ul. Stefanowskiego 4/10, 90-924 Łódź

author

Tomasz Florczak

• Institute of Technical Biochemistry, Lodz University of Technology ul. Stefanowskiego 4/10, 90-924 Łódź

author

Mirosława Szczęsna-Antczak

• Institute of Technical Biochemistry, Lodz University of Technology ul. Stefanowskiego 4/10, 90-924 Łódź

author

Tadeusz Antczak

• Institute of Technical Biochemistry, Lodz University of Technology ul. Stefanowskiego 4/10, 90-924 Łódź

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• Document Type: article