n vitro Antileishmanial Activity of Hydroalcoholic Extract, Fractions, and Compounds Isolated from Leaves of Piper ovatum Vahl against Leishmania amazonensis

• Authors: Daniel RODRIGUES-SILVA , Celso Vataru NAKAMURA , Benedito Prado DIAS FILHO , Tânia UEDA-NAKAMURA , Diógenes Aparício Garcia CORTEZ
• Languages of publication: PL

Abstracts

PL

We assessed the biological activity of a crude extract, a mixture of several fractions, and a pure compound obtained from Piper ovatum Vahl against promastigote and amastigote forms of Leishmania amazonensis. The medicinal plant P. ovatum is used popularly as an anesthetic and anti-inflammatory. This study included the extraction process and bioassay-guided fractionation by the adsorption chromatography and Sephadex LH-20 method. A progressive increase in the antileishmanial effect was observed in the course of fractionation. The 50% inhibitory concentration (IC50) for dichloromethane-ethyl acetate (1:1 v/v) fraction was 2.1 μg/ml and 24 μg/ml; mixture of piperovatine: piperlongumune (2:3) 0.9 μg/ml and 24 μg/ml; piperovatine (1) 9.5 μg/ml and 10 μg/ml; and piperlonguminine (2) 2.5 μg/ml and 9.0 μg/ml, for promastigote and amastigote forms, respectively. Cytotoxicity analysis indicated that these toxic concentrations were much higher for J774G8 macrophages and Vero cells than for the protozoans. The mixture of piperovatine: piperlongumune (2:3) showed important antiprotozoal activity against the amastigote and promastigote forms of L. amazonensis, and it produced morphological changes in promastigotes and amastigotes at 0.9 μg/ml and 24 μg/ml (50% growth inhibition concentration), respectively, including intense cytoplasmic vacuolization, mitochondrial swelling, and mitochondrial damage, as revealed by transmission electron microscopy.

Keywords

PL

Antiprotozoan activity, ultrastructure alterations, Leishmania amazonensis, Piper ovatum

• Publisher: Uniwersytet Jagielloński
• Journal: Acta Protozoologica
• Year: 2009
• Volume: 48
• Issue: 1

Dates

published

2009

online

25 - 09 - 2015

Contributors

author

Daniel RODRIGUES-SILVA

author

Celso Vataru NAKAMURA

author

Benedito Prado DIAS FILHO

author

Tânia UEDA-NAKAMURA

author

Diógenes Aparício Garcia CORTEZ