Analysis of protein posttranslational modifications using mass spectrometry

• Authors: L. Marczak
• Languages of publication: PL

Abstracts

EN

Identification of posttranslational modifications (PTMs) of proteins provides better understanding of their biological functions. Mass spectrometry has become a method of choice for the analysis of PTMs, both MALDI-ToF and tandem (MS/MS) mass spectrometers are perfect tools for assignment of PTMs. The latter due to their peptide fragmentation capability give better identification and information about localization of PTMs in protein molecule. In this paper, an overview of possible use of mass spectrometry and bioinformatic tools together with description of the basic features and limits of the analysis of post translational modifications by mass spectrometry are presented.

Keywords

EN

CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDE FRAGMENTATION; PROTEIN; PROTEOMICS

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2009
• Issue: 2
• Pages: 27-38

Contributors

author

L. Marczak

• Document Type: ARTICLE
• Publication order reference: Lukasz Marczak, Instytut Chemii Bioorganicznej, Polska Akademia Nauk, ul. Noskowskiego 12/14, Poznan, Poland