Comparison of proliferation and motile activity between human keratinocytes isolated from skin and oral muco

• Authors: J. Drukala , J. Zarzecka , K. Gojniczek , A. Waligorska , J. Zapala , W. Korohoda
• Languages of publication: EN

Abstracts

EN

Objective: Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. For healing of skin wounds the skin keratinocytes can be replaced by oral mucosa epithelial cells grown in vitro. The presented experiments were carried out in order to compare the proliferation, morphology, and migration between human keratinocytes isolated from human skin and oral mucosa. Materials and methods: Human epidermal and oral mucosa keratinocytes from primary culture were used in all experiments. Cell motility and shape were determined using computer-aided methods. Results and conclusions: It was demonstrated that although both cell types exhibit the same typical epithelial morphology, oral mucosa keratinocytes locomote significantly faster than skin keratinocytes. They also differ in proliferation activity. Oral mucosa keratinocytes exhibited faster growth and different actin cytoskeleton organisation than skin keratinocytes under in vitro conditions. Autologous oral mucosa keratinocytes may be expanded in vitro and used for skin wound healing in vivo.

Keywords

EN

CELL MIGRATION; KERATINOCYTE; ORAL; PROLIFERATION; SKIN

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
• Journal: Folia Biologica
• Year: 2005
• Volume: 53
• Issue: 1-2
• Pages: 21-28

Contributors

author

J. Drukala

author

J. Zarzecka

author

K. Gojniczek

author

A. Waligorska

author

J. Zapala

author

W. Korohoda

• Document Type: ARTICLE
• Publication order reference: Justyna Drukala, Laboratory of Cell and Tissue Engineering, Department of Cell Biology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland