Hairy root culture of Centaurium erythraea Rafn.

• Authors: E. Piatczak , M. Wielanek , A. Krolicka , E. Lojkowska , H. Wysokinska
• Languages of publication: PL

Abstracts

EN

In this study, the transformed root culture of Centaurium erythraea was established, as a result of infection of leaves culture with agropine strain of Agrobacterium rhizogenes (LBA 9402). Frequency of root formation depended on the kind of explants and the presence of acetosyringone in the bacteria medium. The transformation was confirmed according to the analysis of opines and RAPD-PCR. As the result of transformation 30 clones of hairy roots were obtained. These clones have grown in liquid Woody Plant (WPM) medium without growth regulators. The curve of growth indicates that fresh weight of roots increased almost 17 times , and dry weight increased 12 times during 55 days. Hairy roots of C. erythraea were able to produce secoiridoid glycosides (sweroside, gentiopicroside, swertiamarine).

Keywords

EN

AGROBACTERIUM RHIZOGENES; CENTAURIUM ERYTHRAEA; HAIRY ROOT; SECOIRIDOID

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 2
• Pages: 29-36

Contributors

author

E. Piatczak

author

M. Wielanek

author

A. Krolicka

author

E. Lojkowska

author

H. Wysokinska

• Document Type: ARTICLE
• Publication order reference: E. Piatczak, Zaklad Biologii i Botaniki Farmaceutycznej, Uniwersytet Medyczny, ul. Muszynskiego 1, 90-151 Lodz, Poland