The effect of concentration of mineral salts, kind of solidifying agent, cytokinin and TIBA on adventitious regeneration of apple rootstock P.59

• Authors: K. Wiejacha , T. Orlikowska
• Languages of publication: PL

Abstracts

EN

The aim of this investigation was to establish the conditions for effective regeneration of adventitious shoots on leaf explants of apple dwarf rootstock P.59. A three stage procedure was used ? the induction on medium containing thidiazuron (TDZ) and -naphtalene acetic acid (NAA) for two weeks, then the regeneration on medium supplemented with 6-benzylaminopurine (BAP) and NAA and growth of adventitious shoots on medium with low concentration of BAP and indole-3-butyric acid (IBA). The youngest leaves from proliferating shoot cultures were taken as explants for adventitious regeneration. The effect of MS salt concentration and type of solidifying agent in the induction and regeneration media, and a type of cytokinin in the induction medium was studied. It was proved that the regeneration potential was higher when 1/2 MS salts, Gelrite and TDZ was used.

Keywords

EN

APPLE; CYTOKININ; MINERAL SALT; ROOTSTOCK

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 2
• Pages: 191-198

Contributors

author

K. Wiejacha

author

T. Orlikowska

• Document Type: ARTICLE
• Publication order reference: T. Orlikowska, Instytut Sadownictwa i Kwiaciarstwa, ul. Pomologiczna 18, 96-100 Skierniewice, Poland