Regulation of gene expression in plasmid vectors: doxocyclin-dependent and hypoxia-regulated systems

• Authors: S. Golda , P. Kucharzewska , J. Cisowski , U. Florczyk , A. Zagorska , A. Jazwa , A. Loboda , A. Jozkowicz , J. Dulak
• Languages of publication: PL

Abstracts

EN

Regulation of gene expression in gene therapy is crucial for obtaining the therapeutic effects, thanks to limitation of transgene activity to the selected cells in a given time. In this paper we have focused on plasmid expression systems regulated by doxycycline or hypoxia. We have described in details the structure, regulatory elements and biological applications of 1) the modified, commercially available Tet-On system, expressing doxycycline-controlled b-galactosidase and, 2) hypoxia-activated FGF-4/VEGF expression plasmid containing the hypoxia responsive sequence. The presented expression systems can also be used in viral vectors, enabling not only regulated, but also high and long-term expression of transgenes.

Keywords

EN

GENE EXPRESSION; GENE THERAPY; HYPOXIA

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2007
• Issue: 3
• Pages: 82-97

Contributors

author

S. Golda

author

P. Kucharzewska

author

J. Cisowski

author

U. Florczyk

author

A. Zagorska

author

A. Jazwa

author

A. Loboda

author

A. Jozkowicz

author

J. Dulak

• Document Type: ARTICLE
• Publication order reference: Jozef Dulak, Zaklad Biotechnologii Medycznej, Wydzial Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagiellonski, ul. Gronostajowa 7, 31-271 Krakow, Poland