Evaluation of MR relaxation times following trastuzumab treatment of breast cancer cells in a 3D bioreactor

• Authors: Dorota Bartusik-Aebisher , David Aebisher , Anna Czmil , Damian Mazur
• Languages of publication: EN

Abstracts

EN

The three dimensional tumor environment can be mimicked with the use of cells cultured within a hollow-fiber bioreactor (HFBR). In this work, magnetic resonance imaging (MRI) was used to monitor changes in relaxation time in breast cancer cells following treatment with Trastuzumab in a HFBR system. Breast cancer cells were inoculated into the HFBR system and cultured over a period of 4 weeks. Relaxation time maps were generated according to MRI techniques in three dimensional (3D) cultures of MCF7/Her2 breast cancer and MCF7/Neo4 control cells. MRI measurements showed a variation in values of spin-lattice (T1) and spin-spin (T2) relaxation times related to cell density. Differences in both values (T1 and T2) were noted between untreated cells and cells treated with trastuzumab in the HFBR device. Additionally, 1H MRI was able to provide information about drug penetration in breast cancer cell culture organized in 3D. In conclusion, MRI in vitro can provide direct, noninvasive visualization of cell density in 3D geometry and cell viability as a function of drug uptake. Both T1 and T2 values were higher for lower cell density and accordingly both values, T1 and T2, were lower for higher cell density.

Keywords

EN

magnetic resonance imaging (MRI); breast cancer cell cultures; hollow-fiber bioreactor (HFBR); relaxation time maps

• Journal: Acta Poloniae Pharmaceutica - Drug Research
• Year: 2020
• Volume: 77
• Issue: 1
• Pages: 35-41

Dates

published

2020-02-29

Contributors

author

Dorota Bartusik-Aebisher

author

David Aebisher

author

Anna Czmil

author

Damian Mazur

Identifiers

DOI

10.32383/appdr/115519