Characterization of Fe_3O_4 Magnetic Nanoparticles Modified with Dextran and Investigation of Their Interaction with Protein Amyloid Aggregates

• Authors: M. Koneracká , A. Antošová , V. Závišová , G. Lancz , Z. Gažová , K. Šipošová , A. Juríková , K. Csach , J. Kováč , N. Tomašovičová , M. Fabián , P. Kopčanský
• Languages of publication: EN

Abstracts

EN

Functionalised magnetic nanoparticles composed of Fe_3O_4 particles stabilised by sodium oleate and subsequently modified with dextran (MFDEX) were prepared by the co-precipitation method. Their morphology and particle size distribution were observed by scanning electron microscopy and photon cross correlation spectroscopy. In order to confirm the modification of magnetite surface with dextran physical techniques, including infrared spectroscopy, thermal analysis, and magnetic measurement, were used. Finally, the effect of MFDEX on amyloid fibrillar aggregates of human insulin and hen egg white lysozyme, typical amyloidogenic proteins, was investigated. In vitro interaction of MFDEX with protein amyloid fibrils resulted into destruction of amyloid aggregates. The anti-amyloid activity makes MFDEX of potential interest as therapeutic agent against amyloid-related diseases.

Keywords

EN

75.50.Mm; 87.14.em; 87.19.xr

Discipline

• 87.14.em: Fibrils (amyloids, collagen, etc.)
• 87.19.xr: Degenerative diseases (Alzheimer's, ALS, etc)
• 75.50.Mm: Magnetic liquids

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2010
• Volume: 118
• Issue: 5
• Pages: 983-985

Dates

published

2010-11

Contributors

author

M. Koneracká

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

A. Antošová

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

V. Závišová

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

G. Lancz

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

Z. Gažová

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

K. Šipošová

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia
• Department of Biochemistry, Faculty of Science, Šafárik University, Moyzesova 11, 040 01 Košice, Slovakia

author

A. Juríková

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

K. Csach

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

J. Kováč

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

N. Tomašovičová

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

author

M. Fabián

• Institute of Geotechnics, SAS, Watsonova 45, 040 01 Košice, Slovakia

author

P. Kopčanský

• Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia

References

• 1. A. Bellová, E. Bystrenová, M. Koneracká, P. Kopčanský, F. Valle, M. Timko, J. Bágel'ová, F. Biscarini, Z. Gažová, Nanotechnology 21, 065103 (2010)
• 2. J.D. Sipe, Amyloid Proteins, Wiley-VCH, Weinheim 2005 and references inside
• 3. V. Závišová, M. Koneracká, O. Štrbák, N. Tomašovičová, P. Kopčanský, M. Timko, I. Vávra, J. Magn. Magn. Mater. 311, 379 (2007)
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• 8. S.S.-S. Wang, K.-N. Liu, W.-H. Lee, Biophys. Chem. 144, 78 (2009)

Identifiers

DOI

10.12693/APhysPolA.118.983