Ellipsometrical and SEM Study of Ferritin Adsorption οn a Gold Surface

• Authors: L. Vladimirova , G. Tsutsumanova , Al. Gritzkow , V. Savov
• Languages of publication: EN

Abstracts

EN

The adsorption of horse spleen ferritin on gold surface is studied with null ellipsometry and scanning electron microscopy. The high sensitivity and in situ advantage of ellipsometry make it very attractive for studies of ultrathin biomolecular layers. The influence of pH and protein concentration in solution on the adsorption kinetics was interpreted using ex situ scanning electron microscopy shots and computer modeling of the system "prism-gold film-adsorbed layer-solution". It seems that the first fast stage comprises nucleation process occurring at active sites and the following slow multistage process of a protein film growth. The data about adsorption kinetics of protein and the structure of the adsorbate layers obtained in this study enable understanding of such processes in real biological system, e.g. the protein adsorption on blood vessels and tissues in pathological processes.

Keywords

EN

79.60.Dp; 87.64.Ee; 87.14.Df; 82.45.Jm

Discipline

• 79.60.Dp: Adsorbed layers and thin films
• 87.64.Ee: Electron microscopy
• 87.14.Df: Carbohydrates

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2009
• Volume: 116
• Issue: 4
• Pages: 705-707

Dates

published

2009-10

Contributors

author

L. Vladimirova

• Faculty of Physics, Department of Atomic Physics, Sofia University "St. Kliment Ohridski", 5 James Boucher Blvd., 1164 Sofia, Bulgaria

author

G. Tsutsumanova

• Faculty of Physics, Department of Solid State Physics and Microelectronics, Sofia University "St. Kliment Ohridski", 5 James Boucher Blvd., 1164 Sofia, Bulgaria

author

Al. Gritzkow

• Faculty of Physics, Department of Optics, Sofia University "St. Kliment Ohridski", 5 James Boucher Blvd. 1164 Sofia, Bulgaria

author

V. Savov

• Faculty of Physics, Department of Atomic Physics, Sofia University "St. Kliment Ohridski", 5 James Boucher Blvd., 1164 Sofia, Bulgaria

References

• 1. M. Wahlgren, T. Arnebrant, Trends Biotechnol. 9, 2001 (1991)
• 2. Surface and Interfacial Aspects of Biomedical Polymers, Vol. 2, Protein Adsorption, Ed. J.D. Andrade, Plenum Press, New York 1985
• 3. J.D. Andare, V. Hlady, Adv. Polym. Sci. 70, 1 (1986)
• 4. B. Ivansson, I. Lundström, CDC Crit. Rev. Biocomp. 2, 1 (1986)
• 5. H. Nygren, H. Arwin, S. Welin-Klinström, Colloids Surf. A Physicochem. Eng. Asp. 76, 87 (1993)
• 6. H. Nygren, Biophys. J. 65, 1508 (1994)
• 7. D.R. Weaver, W.G. Pitt, Biomaterials 13, 577 (1992)
• 8. H.D. Jennissen, J. Chromatogr. 159, 71 (1978)
• 9. I. Lundström, H. Elwing, J. Colloid Interface Sci. 136, 68 (1990)

Identifiers

DOI

10.12693/APhysPolA.116.705