EN
This work presents the final development stage and optimization results of the single proton hit facility in Cracow. The setup parameters: proton beam energy 2 MeV, hit resolution 30μm, intensity ≈1000 proton/s, proton registration efficiency 100%, blanking efficiency 99.8%. On-line observation allows targeting cells inside a Petri dish mounted at a 3D precise moving stage. To target a particular cell a semiautomatic cell visualization and recognition system is used.