Study of Probes and Substrates for Low Temperature Atomic Force Microscopy and Biological Applications

• Authors: A. Radenovic , E. Bystrenova , L. Libioulle , G. Dietler
• Languages of publication: EN

Abstracts

EN

The atomic force microscopy in ultrahigh vacuum and at low temperature demonstrated its excellent capability to reach atomic resolution. Nevertheless in the case of biological samples high resolution has been achieved only in very few cases. We demonstrated here the importance of the appropriate choice of probes and substrates in order to image DNA at low temperature with high resolution. We investigated properties of three types of cantilevers and they were studied by scanning electron microscopy as a function of temperature. A large bending of cantilevers, which were coated from both sides, was observed at low temperatures. Therefore uncoated cantilevers are strongly recommended for low temperature applications. Different methods for immobilization of DNA on the substrate are examined at low temperatures. First images of linear DNA on graphite at 82 K under ultrahigh vacuum conditions are presented.

Keywords

EN

07.79.Lh; 68.37.Hk; 82.39.Pj; 07.20.Mc

Discipline

• 07.20.Mc: Cryogenics; refrigerators, low-temperature detectors, and other low-temperature equipment
• 68.37.Hk: Scanning electron microscopy (SEM) (including EBIC)
• 07.79.Lh: Atomic force microscopes
• 82.39.Pj: Nucleic acids, DNA and RNA bases(for DNA, see 87.14.gk; for RNA, see 87.14.gn)

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2003
• Volume: 104
• Issue: 3-4
• Pages: 373-380

Dates

published

2003-09/10

received

2003-07-16

Contributors

author

A. Radenovic

• Institute of Condensed Matter Physics, University of Lausanne, 1015 Lausanne, Switzerland

author

E. Bystrenova

• Institute of Condensed Matter Physics, University of Lausanne, 1015 Lausanne, Switzerland

author

L. Libioulle

• Institute of Condensed Matter Physics, University of Lausanne, 1015 Lausanne, Switzerland

author

G. Dietler

• Institute of Condensed Matter Physics, University of Lausanne, 1015 Lausanne, Switzerland

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Identifiers

DOI

10.12693/APhysPolA.104.373