Ability of Lactobacillus plantarum LS/07 to modify intestinal enzymes activity in chronic diseases prevention

• Authors: Emília Hijová , Jozef Kuzma , Ladislav Strojný , Alojz Bomba , Izabela Bertková , Anna Chmelárová , Zdena Hertelyová , Veronika Benetinová , Jana Štofilová , Ľuboš Ambro
• Languages of publication: EN

Abstracts

EN

The ability of probiotic strain Lactobacillus plantarum LS/07 to modify the activity of intestinal bacterial enzymes - β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in prevention of chronic diseases - cancer, atherosclerosis and dysbiosis was investigated. The male Sprague-Dawley rats were randomly divided into 12 experimental groups: controls groups - C (control), AT (atherosclerotic), CC (carcinogenic), dysbiotic groups - each group in combination with antibiotics (ATB), probiotics groups - in combinatioan with probiotic (PRO) alone, and each group with combination of antibiotic and probiotic (ATB+PRO). In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in atherosclerotic group significantly increased the activity of β-glucuronidase (P<0.001) and β-glucosidase (P<0.01). Azoxymethane application in carcinogenic group significantly increased β-glucuronidase (P<0.01), but reduced β-glucosidase (P<0.01) activity. Daily application of probiotics alone and in combination with antibiotic increased β-galactosidase, of β-glucosidase, and decreased β-glucuronidase activity. In control antibiotic group we observed significant increase in β-glucuronidase (P<0.05) and decreased β-glucosidase (P<0.01) activity which can be caused by the change of microflora in favor of coliform bacteria. These findings indicate the positive effects of probiotic Lactobacillus plantarum LS/07 and suggest its use in disease prevention in human medicine and some animal species.

Keywords

EN

Sprague-Dawley rats; bacterial enzyme; cancer; atherosclerosis; dysbiosis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 1
• Pages: 113-116

Dates

published

2017

received

2016-05-04

revised

2016-07-14

accepted

2016-09-09

(unknown)

2016-11-07

Contributors

author

Emília Hijová

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Jozef Kuzma

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Ladislav Strojný

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Alojz Bomba

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Izabela Bertková

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Anna Chmelárová

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Zdena Hertelyová

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Veronika Benetinová

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Jana Štofilová

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

author

Ľuboš Ambro

• Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic

References

• An HM, Park SY, Lee DK, Kim JR, Cha MK, Lee SW, Lim HT, Kim KJ, Ha NJ (2011) Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats. Lipids Health Dis 10: 116. doi: 10.1186/1476-511X-10-116.
• Arthur JC, Jobin C (2011) The struggle within: microbial influences in colorectal cancer. Inflamm Bowel Dis 17: 396-409. doi: 10.1002/ibd.21354.
• Bertkova I, Hijova E, Chmelarova A, Mojzisova G, Petrasova D, Strojny L, Bomba A, Zitnan R (2010) The effect of probiotic microorganisms and bioactive compounds on chemically induced carcinogenesis in rats. Neoplasma 57: 422-428. doi: 10.4149/neo_2010_05_422.
• Blaut M, Clavel T (2007) Metabolic diversity of the intestinal microbiota: implications for health and disease. J Nutr 137: 751S-755S.
• Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, Delzenne NM, Burcelin R (2008) Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 57: 1470-1481. doi: 10.2337/db07-1403.
• Dabek M, McCrae SI, Stevens VJ, Duncan SH, Louis P (2008) Distribution of β-glucosidase and β-glucuronidase activity and of β-glucuronidase gene gus in human colonic bacteria. FEMS Microbiol Ecol 66: 487-495. doi: 10.1111/j.1574-6941.2008.00520.x.
• De Preter V, Raemen H, Cloetens L, Houben E, Rutgeerts P, Verbeke K (2008) Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities. Eur J Clin Nutr 62: 225-231. doi: 10.1038/sj.ejcn.1602706.
• Gill CI, Rowland IR (2002) Diet and cancer: assessing the risk. Br J Nutr 88: S73-S87. See comment in PubMed Commons below.
• Hijová E, Šoltésová A, Salaj R, Kuzma J, Strojný L, Bomba A (2015) Use of the prebiotic inulin in the prevention of adverse signs of acute colitis. Acta Veterinaria-Beograd 65: 339-347. doi: 10.1515/acve-2015-0028
• Juskiewicz J, Zdunczyk Z, Wroblewska M, Oszmianski J, Hernandez T (2002) The responce of rats to feeding with diets containing grapefruit flavonoid extract. Food Res Int 35: 201-205. doi: 10.1016/S0963-9969(01)00184-3
• Kim DH (2015) Gut microbiota-mediated drug-antibiotic interactions. Drug Metab Dispos 43: 1581-1589. doi: 10.1124/dmd.115.063867.
• McBain AJ, MacFarlane GT (1998) Ecological and physiological studies on large intestinal bacteria in relation to production of hydrolytic and reductive enzymes involved in formation of genotoxic metabolites. J Med Microbiol 47: 407-416. doi: 10.1099/00222615-47-5-407.
• Michlmayr H, Kneifel W (2014) β-Glucosidase activities of lactic acid bacteria: mechanisms, impact on fermented food and human health. FEMS Microbiol Lett 352: 1-10. doi: 10.111/1574-6968.12348.
• Mroczynska M, Libudzisz Z (2010) β-Glucuronidase and β-Glucosidase activity of lactobacillus and Enterococcus isolated from human feces. Pol J Microbiol 59: 265-269.
• Nakamura J, Kubota Y, Miyaoka M, Saitoh T, Mizuno F, Benno Y (2002) Comparison of four microbial enzymes in Clostridia and Bacteroides isolated from human feces. Microbiol Immunol 46: 487-490. doi: 10.1111/j.1348-0421.2002.tb02723.x.
• Panwar H, Calderwood D, Grant IR, Grover S, Green BD (2014) Lactobacillus strains isolated from infant faeces possess potent inhibitory activity against alpha- and beta-glucosidases suggesting anti-diabetic potential. Eur J Nutr 53: 1465-1474. doi: 10.1007/s00394-013-0649-9.
• Plotnikoff GA (2014) Three measurable and modifiable enteric microbial biotransformations relevant to cancer prevention and treatment. Glob Adv Health Med 3: 33-43. doi: 10.7453/gahmj.2014.021.
• Rowland IR, Rumney CJ, Coutts JT, Lievense LC (1998) Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats. Carcinogenesis 19: 281-285.
• Sobhani J, Nhieu JT (2013) Colon cancer is associated with microbial dysbiosis in humans and animals. Govaresh 18: 45-56.
• Strojný L, Bomba A, Hijová E, Chmelárová A, Mojžišová G, Bertková I, Koprovičová J, Pomfy M, Strompfová V, Molokáčová M (2011) Effects of probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats. Czech J Anim Sci 56: 99-106.

Identifiers

DOI

10.18388/abp.2016_1308