Barley primary microRNA expression pattern is affected by soil water availability

• Authors: Aleksandra Swida-Barteczka , Katarzyna Kruszka , Aleksandra Grabowska , Andrzej Pacak , Artur Jarmolowski , Marzena Kurowska , Iwona Szarejko , Zofia Szweykowska-Kulinska
• Languages of publication: EN

Abstracts

EN

MicroRNAs are short molecules of 21-24 nt in length. They are present in all eukaryotic organisms and regulate gene expression by guiding posttranscriptional silencing of mRNAs. In plants, they are key players in signal transduction, growth and development, and in response to abiotic and biotic stresses. Barley (Hordeum vulgare) is an economically important monocotyledonous crop plant. Drought is the world's main cause of loss in cereal production. We have constructed a high-throughput Real-Time RT-qPCR platform for parallel determination of 159 barley primary microRNAs' levels. The platform was tested for two drought-and-rehydration-treated barley genotypes (Rolap and Sebastian). We have determined changes in the expression of primary microRNAs responding to mild drought, severe drought, and rehydration. Based on the results obtained, we conclude that alteration in the primary microRNA expression is relative to the stress's intensity. Mild drought and rehydration mostly decrease the pri-miRNA levels in both of the tested genotypes. Severe drought mainly induces the primary microRNA expression. The main difference between the genotypes tested was a much-stronger induction of pri-miRNAs in Rolap encountering severe drought. The primary microRNAs respond dynamically to mild drought, severe drought, and rehydration treatments. We propose that some of the individual pri-miRNAs could be used as drought stress or rehydration markers. The usage of the platform in biotechnology is also postulated.

Keywords

EN

pri-miRNA; miRNA; drought; rehydration; barley genotypes

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 4
• Pages: 817-824

Dates

published

2016

received

2016-06-03

revised

2016-06-30

accepted

2016-07-20

(unknown)

2016-10-21

Contributors

author

Aleksandra Swida-Barteczka

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland

author

Katarzyna Kruszka

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland

author

Aleksandra Grabowska

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland

author

Andrzej Pacak

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland

author

Artur Jarmolowski

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland

author

Marzena Kurowska

• Department of Genetics, Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland

author

Iwona Szarejko

• Department of Genetics, Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland

author

Zofia Szweykowska-Kulinska

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland

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Identifiers

DOI

10.18388/abp.2016_1352