Diverse expression of N-acetylglucosaminyltransferase V and complex-type β1,6-branched N-glycans in uveal and cutaneous melanoma cells

• Authors: Ewa Pocheć , Magdalena Rydlewska , Małgorzata Przybyło , Anna Lityńska
• Languages of publication: EN

Abstracts

EN

Although both uveal (UM) and cutaneous (CM) melanoma cells derive from the transformed melanocytes, their biology varies significantly in several aspects. Malignant transformation is frequently associated with alternations in cell glycosylation, in particular those concerning branched complex-type N-glycans. These changes occur principally in β1,4-N-acetylglucosaminyltransferase III (GnT-III) that catalyzes the synthesis of glycans with bisected N-acetylglucosamine (GlcNAc) and β1,6-N-acetylglucosaminyltransferase V (GnT-V) that is involved in forming β1,6-branched antenna in complex-type glycans. We searched for the reasons of a different behavior of CM and UM cells in the expression of GnT-III and GnT-V and their oligosaccharide products. Our study showed that UM cells have more β1,6-branched glycans than CM cells, what results from a higher expression of MGAT5 gene encoding GnT-V. The higher β1,6-branching of glycans in UM may contribute to their higher potential to migrate on fibronectin and weaker binding to main extracellular matrix proteins, observed in our previous studies.

Keywords

EN

β1,4-N-acetylglucosaminyltransferase III; β1,6-N-acetylglucosaminyltransferase V; bisected GlcNAc; β1,6-branched N-glycans; uveal melanoma; cutaneous melanoma

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 2
• Pages: 323-328

Dates

published

2015

received

2015-05-07

revised

2015-05-25

accepted

2015-06-19

(unknown)

2015-06-22

Contributors

author

Ewa Pocheć

• Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Magdalena Rydlewska

• Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Małgorzata Przybyło

• Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Anna Lityńska

• Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, Kraków, Poland

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Identifiers

DOI

10.18388/abp.2015_1050