Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold

• Authors: Tobiasz Deptuła , Alicja Warowicka , Anna Woźniak , Mikołaj Grzeszkowiak , Maciej Jarzębski , Magdalena Bednarowicz , Adam Patkowski , Ryszard Słomski
• Languages of publication: EN

Abstracts

EN

Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. Additionally, we report that the pNiPAM nanoparticle based scaffold promotes cell growth.

Keywords

EN

nanoparticles; toxicology; poly-N-isopropylacrylamide; drug delivery system; cytotoxicity; polymers

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 2
• Pages: 311-316

Dates

published

2015

received

2015-03-12

revised

2015-05-02

accepted

2015-05-06

(unknown)

2015-05-18

Contributors

author

Tobiasz Deptuła

• Molecular Biophysics Division, Adam Mickiewicz University, Faculty of Physics, Poznań, Poland
• NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland

author

Alicja Warowicka

• NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland

author

Anna Woźniak

• NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland

author

Mikołaj Grzeszkowiak

• NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
• Division of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Poland

author

Maciej Jarzębski

• Molecular Biophysics Division, Adam Mickiewicz University, Faculty of Physics, Poznań, Poland
• NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland

author

Magdalena Bednarowicz

• NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland

author

Adam Patkowski

• Molecular Biophysics Division, Adam Mickiewicz University, Faculty of Physics, Poznań, Poland
• NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland

author

Ryszard Słomski

• NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland
• Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznań, Poland

References

• Abu Samah NH, Heard CM (2014) The effects of topically applied polyNIPAM-based nanogels and their monomers on skin cyclooxygenase expression, ex vivo. Nanotoxicology 8: 100-106.
• Akiyama Y, Kikuchi A, Yamato M, Okano T (2004) Ultrathin poly(N-isopropylacrylamide) grafted layer on polystyrene surfaces for cell adhesion/detachment control. Langmuir 20: 5506-5511.
• Alaghemandi M, Spohr E (2013) A molecular dynamics study of poly(N-isopropylacrylamide) endgrafted on a model cylindrical pore surface. RSC Advances 3: 3638-3647.
• Cooperstein MA, Canavan HE (2010) Biological cell detachment from poly(N-isopropyl acrylamide) and its applications. Langmuir 26: 7695-7707.
• Cooperstein MA, Canavan HE (2013) Assessment of cytotoxicity of (N-isopropyl acrylamide) and poly(N-isopropyl acrylamide)-coated surfaces. Biointerphases 8: 19.
• Gil ES, Hudson SM (2004) Stimuli-reponsive polymers and their bioconjugates. Progress in Polymer Science 29: 1173-1222.
• Hoffman AS (1995) Intelligent polymers in medicine and biotechnology. Artif Organs 19: 458-467.
• Huber DL, Manginell RP, Samara MA, Kim BI, Bunker BC (2003) Programmed adsorption and release of proteins in a microfluidic device. Science 301: 352-354.
• Hussien R, Rihn BH, Eidi H, Ronzani C, Joubert O, Ferrari L, Vazquez O, Kaufer D, Brooks GA (2013) Unique growth pattern of human mammary epithelial cells induced by polymeric nanoparticles. Physiol Rep 1: e00027.
• Kanazawa H, Yamamoto K, Matsushima Y, Takai N, Kikuchi A, Sakurai Y, Okano T (1996) Temperature-responsive chromatography using poly(N-isopropylacrylamide)-modified silica. Analytical Chemistry 68: 100-105.
• Lien YH, Wu TM, Wu JH, Liao JW (2011) Cytotoxicity and drug release behavior of PNIPAM grafted on silica-coated iron oxide nanoparticles. J Nanoparticle Res 13: 5065-5075.
• Mandal K, Balland M, Bureau L (2012) Thermoresponsive micropatterned substrates for single cell studies. PLoS ONE 7: e37548.
• Mansfield E, Oreskovic TL, Rentz NS, Jeerage KM (2014) Three-dimensional hydrogel constructs for exposing cells to nanoparticles. Nanotoxicology 8: 394-403.
• Meenach SA, Anderson AA, Suthar M, Anderson KW, Hilt JZ (2009) Biocompatibility analysis of magnetic hydrogel nanocomposites based on poly(N-isopropylacrylamide) and iron oxide. J Biomed Mater Res A 91: 903-909.
• Naha PC, Bhattacharya K, Tenuta T, Dawson KA, Lynch I, Gracia A, Lyng FM, Byrne HJ (2010) Intracellular localisation, geno- and cytotoxic response of polyN-isopropylacrylamide (PNIPAM) nanoparticles to human keratinocyte (HaCaT) and colon cells (SW 480). Toxicol Lett 198: 134-143.
• Ravichandran R (2009) Nanotechnology based drug delivery system. NanoBiotechnnology 5: 17-33.
• Shimizu H, Wada R Okabe M (2009) Preparation and characterization of micrometer-sized poly(N-isopropylacrylamide) hydrogel particles. Polym J 41: 771-777.
• Vihola H, Laukkanen A, Valtola L, Tenhu H, Hirvonen J (2005) Cytotoxicity of thermosensitive polymers poly(N-isopropylacrylamide), poly(N-vinylcaprolactam) and amphiphilically modified poly(N-vinylcaprolactam). Biomaterials 26: 3055-3064.
• Wadajkar A, Koppolu B, Rahimi M, Nguyen K (2009) Cytotoxic evaluation of N-isopropylacrylamide monomers and temperature-sensitive poly(N-isopropylacrylamide) nanoparticles. J Nanoparticle Res 11: 1375-1382.
• Xu FJ, Zheng YQ, Zhen WJ, Yang WT (2011) Thermoresponsive poly(N-isopropyl acrylamide)-grafted polycaprolactone films with surface immobilization of collagen. Colloids Surf B Biointerfaces 85: 40-47.

Identifiers

DOI

10.18388/abp.2015_1007