Consequences of the loss of the Grainyhead-like 1 gene for renal gene expression, regulation of blood pressure and heart rate in a mouse model

• Authors: Magdalena Pawlak , Agnieszka Walkowska , Michał Mlącki , Jelena Pistolic , Tomasz Wrzesiński , Vladimir Benes , Stephen Jane , Joanna Wesoły , Elżbieta Kompanowska-Jezierska , Tomasz Wilanowski
• Languages of publication: EN

Abstracts

EN

Aim: The Grainyhead-like 1 (GRHL1) transcription factor is tissue-specific and is very highly expressed in the kidney. In humans the GRHL1 gene is located at the chromosomal position 2p25. A locus conferring increased susceptibility to essential hypertension has been mapped to 2p25 in two independent studies, but the causative gene has never been identified. Furthermore, a statistically significant association has been found between a polymorphism in the GRHL1 gene and heart rate regulation. The aim of our study was to investigate the physiological consequences of Grhl1 loss in a mouse model and ascertain whether Grhl1 may be involved in the regulation of blood pressure and heart rate. Experimental approach: In our research we employed the Grhl1 "knock-out" mouse strain. We analyzed renal gene expression, blood pressure and heart rate in the Grhl1-null mice in comparison with their "wild-type" littermate controls. Most important results: The expression of many genes is altered in the Grhl1-/- kidneys. Some of these genes have previously been linked to blood pressure regulation. Despite this, the Grhl1-null mice have normal blood pressure and interestingly, increased heart rate. Conclusions: Our work did not discover any new evidence to suggest any involvement of Grhl1 in blood pressure regulation. However, we determined that the loss of Grhl1 influences the regulation of heart rate in a mouse model.

Keywords

EN

blood pressure; genetics; grainy head; heart rate; kidney; transcription factor

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 2
• Pages: 287-296

Dates

published

2015

received

2015-03-02

revised

2015-03-23

accepted

2015-03-30

(unknown)

2015-04-21

Contributors

author

Magdalena Pawlak

• Laboratory of Signal Transduction, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

author

Agnieszka Walkowska

• Department of Renal and Body Fluid Physiology, Mossakowski Medical Research Center, Polish Academy of Sciences, Warsaw, Poland

author

Michał Mlącki

• Laboratory of Signal Transduction, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

author

Jelena Pistolic

• Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany

author

Tomasz Wrzesiński

• Laboratory of High Throughput Technologies, Institute of Biotechnology and Molecular Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Vladimir Benes

• Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany

author

Stephen Jane

• Department of Medicine, Monash University Central Clinical School, Prahran VIC, Australia
• Alfred Hospital, Prahran VIC, Australia

author

Joanna Wesoły

• Laboratory of High Throughput Technologies, Institute of Biotechnology and Molecular Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Elżbieta Kompanowska-Jezierska

• Department of Renal and Body Fluid Physiology, Mossakowski Medical Research Center, Polish Academy of Sciences, Warsaw, Poland

author

Tomasz Wilanowski

• Laboratory of Signal Transduction, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

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Identifiers

DOI

10.18388/abp.2015_1001