Transcriptional changes between uninflamed ulcerative colitis and familial adenomatous polyposis pouch mucosa can be attributed to an altered immune response

• Authors: Agnieszka Paziewska , Karolina Horbacka , Krzysztof Goryca , Michal Mikula , Dorota Jarosz , Michalina Dabrowska , Piotr Krokowicz , Jacek Karon , Jerzy Ostrowski
• Languages of publication: EN

Abstracts

EN

A total proctocolectomy with ileal pouch-anal anastomosis (IPAA) is considered the surgery of choice for definitive management of familial adenomatous polyposis (FAP) and some patients with ulcerative colitis (UC). However, this surgical treatment is often associated with pouchitis, a long-term complication that occurs mostly in UC patients. The purpose of this study was to better define the molecular background of pouchitis. A microarray-based survey was performed using pouch mucosal samples collected from 28 and 8 patients undergoing surgery for UC and FAP, respectively. There were 4,770 genes that significantly differentiated uninflamed from inflamed mucosal samples, and their functional features were represented mostly by metabolic and cell proliferation pathways. In contrast, functional analyses of aberrantly expressed genes between UC and FAP samples, irrespective of mucosal inflammation status, revealed multiple pathways and terms that were linked to changes in immune response. Interestingly, the comparison of uninflamed UC and FAP samples identified a set of 29 altered probe sets, including an inflammation-related transcript encoding a Charcot-Leyden crystal (CLC) protein. The most distinct changes in gene expression profiles differentiating uninflamed UC and FAP pouch mucosal samples were attributed to the Gene Ontology category innate immune response. Our study confirmed that alterations in immune responses can be found between patients who underwent surgery for UC and FAP, independent of the pouch inflammation status. This observation may be important when managing IPAA patients.

Keywords

EN

ulcerative colitis; familial adenomatous polyposis; pouch; gene expression; immune response

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 1
• Pages: 69-75

Dates

published

2015

received

2014-04-22

revised

2014-11-04

accepted

2014-11-12

online

2015-02-04

Contributors

author

Agnieszka Paziewska

• Department of Gastroenterology and Hepatology, Medical Center for Postgraduate Education, Warsaw, Poland

author

Karolina Horbacka

• Department of General and Colorectal Surgery, Poznan University of Medical Sciences, Poznań, Poland

author

Krzysztof Goryca

• Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland

author

Michal Mikula

• Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland

author

Dorota Jarosz

• Department of Gastroenterology and Hepatology, Medical Center for Postgraduate Education, Warsaw, Poland

author

Michalina Dabrowska

• Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland

author

Piotr Krokowicz

• Department of General and Colorectal Surgery, Poznan University of Medical Sciences, Poznań, Poland

author

Jacek Karon

• Department of General and Colorectal Surgery, Poznan University of Medical Sciences, Poznań, Poland

author

Jerzy Ostrowski

• Department of Gastroenterology and Hepatology, Medical Center for Postgraduate Education, Warsaw, Poland

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