Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification

• Authors: Natalia Kobylak , Barbara Bykowska , Roman Nowicki , Anna Brillowska-Dąbrowska
• Languages of publication: EN

Abstracts

EN

Dermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction method and real-time PCR was used for detection of dermatophytes DNA using pan-dermatophyte primers and identification of Trichophyton rubrum from pure cultures. The applied method allowed correct detection of all dermatophytes and correct identification of Trichophyton rubrum in less than 2 hours.

Keywords

EN

fungal infections; dermatophytosis; DNA extraction; real-time PCR

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 1
• Pages: 119-122

Dates

published

2015

received

2014-07-18

revised

2014-11-13

accepted

2014-11-21

online

2015-02-24

Contributors

author

Natalia Kobylak

• Department of Microbiology, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland

author

Barbara Bykowska

• Department of Dermatology, Venereology and Allergology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland

author

Roman Nowicki

• Department of Dermatology, Venereology and Allergology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland

author

Anna Brillowska-Dąbrowska

• Department of Microbiology, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland

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