Cystain C and neuropeptid Y levels in brain tissues after experimental subarachnoid hemorrhage

• Authors: Şerefden Açıkgöz , Murat Can , Berrak Güven , Nurullah Edebali , Figen Barut , Çağatay Büyükuysal , İshak Tekin , Bektaş Açıkgöz
• Languages of publication: EN

Abstracts

EN

The aim of this study was to investigate the changes in the levels of cystatin C, which protects neurodegeneration in the central nervous system with the inhibition of cysteine protease and by inducing autophagy in the pathogenesis of cerebral vasospasm and levels of vasoconstrictive neuropeptid Y (NPY) in the brain tissue homogenates of rat model of subarachnoid hemorrhage (SAH). Three experimental groups were used: Day 2 and Day 7 groups after SAH, and also a control group. There were seven Wistar albino rats in each group. SAH was accomplished by transclival basilar artery puncture. Rat cystatin C, rat NPY were determined with ELISA in brain tissue homogenates. Day 2 group showed significantly enhanced cystatin C values in comparision with the control group (P=0.048). NPY levels between the Day 2 and Day 7 groups and the control groups were not significantly different (P=0.315). In histopathological examination, there was less neuronal loss in the Day 2 group than in the Day 7 group. Regarding our results, it would be more valuable to measure NPY levels in specific brain areas. The increased cystatin C levels on the second day after SAH is probably a pathophysiologic mechanism to organize protease activity.

Keywords

EN

cystatin C; neuropeptid Y; experimental subarachnoid hemorrhage

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 4
• Pages: 825-828

Dates

published

2014

received

2014-10-24

revised

2014-11-05

accepted

2014-11-13

(unknown)

2014-12-18

Contributors

author

Şerefden Açıkgöz

• Department of Biochemistry, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey

author

Murat Can

• Department of Biochemistry, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey

author

Berrak Güven

• Department of Biochemistry, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey

author

Nurullah Edebali

• Department Neurosurgery, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey

author

Figen Barut

• Department of Pathology, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey

author

Çağatay Büyükuysal

• Department of Biostatistics, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey

author

İshak Tekin

• Department of Immunology Faculty of Medicine, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey

author

Bektaş Açıkgöz

• Department Neurosurgery, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey

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